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Hypothesis Testing – A Complete Guide with Examples

Published by Alvin Nicolas at August 14th, 2021 , Revised On October 26, 2023

In statistics, hypothesis testing is a critical tool. It allows us to make informed decisions about populations based on sample data. Whether you are a researcher trying to prove a scientific point, a marketer analysing A/B test results, or a manufacturer ensuring quality control, hypothesis testing plays a pivotal role. This guide aims to introduce you to the concept and walk you through real-world examples.

What is a Hypothesis and a Hypothesis Testing?

A hypothesis is considered a belief or assumption that has to be accepted, rejected, proved or disproved. In contrast, a research hypothesis is a research question for a researcher that has to be proven correct or incorrect through investigation.

What is Hypothesis Testing?

Hypothesis testing  is a scientific method used for making a decision and drawing conclusions by using a statistical approach. It is used to suggest new ideas by testing theories to know whether or not the sample data supports research. A research hypothesis is a predictive statement that has to be tested using scientific methods that join an independent variable to a dependent variable.  

Example: The academic performance of student A is better than student B

Characteristics of the Hypothesis to be Tested

A hypothesis should be:

• Clear and precise
• Capable of being tested
• Able to relate to a variable
• Stated in simple terms
• Consistent with known facts
• Limited in scope and specific
• Tested in a limited timeframe
• Explain the facts in detail

What is a Null Hypothesis and Alternative Hypothesis?

A  null hypothesis  is a hypothesis when there is no significant relationship between the dependent and the participants’ independent  variables . 

In simple words, it’s a hypothesis that has been put forth but hasn’t been proved as yet. A researcher aims to disprove the theory. The abbreviation “Ho” is used to denote a null hypothesis.

If you want to compare two methods and assume that both methods are equally good, this assumption is considered the null hypothesis.

Example: In an automobile trial, you feel that the new vehicle’s mileage is similar to the previous model of the car, on average. You can write it as: Ho: there is no difference between the mileage of both vehicles. If your findings don’t support your hypothesis and you get opposite results, this outcome will be considered an alternative hypothesis.

If you assume that one method is better than another method, then it’s considered an alternative hypothesis. The alternative hypothesis is the theory that a researcher seeks to prove and is typically denoted by H1 or HA.

If you support a null hypothesis, it means you’re not supporting the alternative hypothesis. Similarly, if you reject a null hypothesis, it means you are recommending the alternative hypothesis.

Example: In an automobile trial, you feel that the new vehicle’s mileage is better than the previous model of the vehicle. You can write it as; Ha: the two vehicles have different mileage. On average/ the fuel consumption of the new vehicle model is better than the previous model.

If a null hypothesis is rejected during the hypothesis test, even if it’s true, then it is considered as a type-I error. On the other hand, if you don’t dismiss a hypothesis, even if it’s false because you could not identify its falseness, it’s considered a type-II error.

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How to Conduct Hypothesis Testing?

Here is a step-by-step guide on how to conduct hypothesis testing.

Step 1: State the Null and Alternative Hypothesis

Once you develop a research hypothesis, it’s important to state it is as a Null hypothesis (Ho) and an Alternative hypothesis (Ha) to test it statistically.

A null hypothesis is a preferred choice as it provides the opportunity to test the theory. In contrast, you can accept the alternative hypothesis when the null hypothesis has been rejected.

Example: You want to identify a relationship between obesity of men and women and the modern living style. You develop a hypothesis that women, on average, gain weight quickly compared to men. Then you write it as: Ho: Women, on average, don’t gain weight quickly compared to men. Ha: Women, on average, gain weight quickly compared to men.

Step 2: Data Collection

Hypothesis testing follows the statistical method, and statistics are all about data. It’s challenging to gather complete information about a specific population you want to study. You need to  gather the data  obtained through a large number of samples from a specific population. 

Example: Suppose you want to test the difference in the rate of obesity between men and women. You should include an equal number of men and women in your sample. Then investigate various aspects such as their lifestyle, eating patterns and profession, and any other variables that may influence average weight. You should also determine your study’s scope, whether it applies to a specific group of population or worldwide population. You can use available information from various places, countries, and regions.

Step 3: Select Appropriate Statistical Test

There are many  types of statistical tests , but we discuss the most two common types below, such as One-sided and two-sided tests.

Note: Your choice of the type of test depends on the purpose of your study 

One-sided Test

In the one-sided test, the values of rejecting a null hypothesis are located in one tail of the probability distribution. The set of values is less or higher than the critical value of the test. It is also called a one-tailed test of significance.

Example: If you want to test that all mangoes in a basket are ripe. You can write it as: Ho: All mangoes in the basket, on average, are ripe. If you find all ripe mangoes in the basket, the null hypothesis you developed will be true.

Two-sided Test

In the two-sided test, the values of rejecting a null hypothesis are located on both tails of the probability distribution. The set of values is less or higher than the first critical value of the test and higher than the second critical value test. It is also called a two-tailed test of significance. 

Example: Nothing can be explicitly said whether all mangoes are ripe in the basket. If you reject the null hypothesis (Ho: All mangoes in the basket, on average, are ripe), then it means all mangoes in the basket are not likely to be ripe. A few mangoes could be raw as well.

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Step 4: Select the Level of Significance

When you reject a null hypothesis, even if it’s true during a statistical hypothesis, it is considered the  significance level . It is the probability of a type one error. The significance should be as minimum as possible to avoid the type-I error, which is considered severe and should be avoided. 

If the significance level is minimum, then it prevents the researchers from false claims. 

The significance level is denoted by  P,  and it has given the value of 0.05 (P=0.05)

If the P-Value is less than 0.05, then the difference will be significant. If the P-value is higher than 0.05, then the difference is non-significant.

Example: Suppose you apply a one-sided test to test whether women gain weight quickly compared to men. You get to know about the average weight between men and women and the factors promoting weight gain.

Step 5: Find out Whether the Null Hypothesis is Rejected or Supported

After conducting a statistical test, you should identify whether your null hypothesis is rejected or accepted based on the test results. It would help if you observed the P-value for this.

Example: If you find the P-value of your test is less than 0.5/5%, then you need to reject your null hypothesis (Ho: Women, on average, don’t gain weight quickly compared to men). On the other hand, if a null hypothesis is rejected, then it means the alternative hypothesis might be true (Ha: Women, on average, gain weight quickly compared to men. If you find your test’s P-value is above 0.5/5%, then it means your null hypothesis is true.

Step 6: Present the Outcomes of your Study

The final step is to present the  outcomes of your study . You need to ensure whether you have met the objectives of your research or not. 

In the discussion section and  conclusion , you can present your findings by using supporting evidence and conclude whether your null hypothesis was rejected or supported.

In the result section, you can summarise your study’s outcomes, including the average difference and P-value of the two groups.

If we talk about the findings, our study your results will be as follows:

Example: In the study of identifying whether women gain weight quickly compared to men, we found the P-value is less than 0.5. Hence, we can reject the null hypothesis (Ho: Women, on average, don’t gain weight quickly than men) and conclude that women may likely gain weight quickly than men.

Did you know in your academic paper you should not mention whether you have accepted or rejected the null hypothesis? 

Always remember that you either conclude to reject Ho in favor of Haor   do not reject Ho . It would help if you never rejected  Ha  or even  accept Ha .

Suppose your null hypothesis is rejected in the hypothesis testing. If you conclude  reject Ho in favor of Haor   do not reject Ho,  then it doesn’t mean that the null hypothesis is true. It only means that there is a lack of evidence against Ho in favour of Ha. If your null hypothesis is not true, then the alternative hypothesis is likely to be true.

Example: We found that the P-value is less than 0.5. Hence, we can conclude reject Ho in favour of Ha (Ho: Women, on average, don’t gain weight quickly than men) reject Ho in favour of Ha. However, rejected in favour of Ha means (Ha: women may likely to gain weight quickly than men)

Frequently Asked Questions

What are the 3 types of hypothesis test.

The 3 types of hypothesis tests are:

• One-Sample Test : Compare sample data to a known population value.
• Two-Sample Test : Compare means between two sample groups.
• ANOVA : Analyze variance among multiple groups to determine significant differences.

What is a hypothesis?

A hypothesis is a proposed explanation or prediction about a phenomenon, often based on observations. It serves as a starting point for research or experimentation, providing a testable statement that can either be supported or refuted through data and analysis. In essence, it’s an educated guess that drives scientific inquiry.

What are null hypothesis?

A null hypothesis (often denoted as H0) suggests that there is no effect or difference in a study or experiment. It represents a default position or status quo. Statistical tests evaluate data to determine if there’s enough evidence to reject this null hypothesis.

What is the probability value?

The probability value, or p-value, is a measure used in statistics to determine the significance of an observed effect. It indicates the probability of obtaining the observed results, or more extreme, if the null hypothesis were true. A small p-value (typically <0.05) suggests evidence against the null hypothesis, warranting its rejection.

What is p value?

The p-value is a fundamental concept in statistical hypothesis testing. It represents the probability of observing a test statistic as extreme, or more so, than the one calculated from sample data, assuming the null hypothesis is true. A low p-value suggests evidence against the null, possibly justifying its rejection.

What is a t test?

A t-test is a statistical test used to compare the means of two groups. It determines if observed differences between the groups are statistically significant or if they likely occurred by chance. Commonly applied in research, there are different t-tests, including independent, paired, and one-sample, tailored to various data scenarios.

When to reject null hypothesis?

Reject the null hypothesis when the test statistic falls into a predefined rejection region or when the p-value is less than the chosen significance level (commonly 0.05). This suggests that the observed data is unlikely under the null hypothesis, indicating evidence for the alternative hypothesis. Always consider the study’s context.

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• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Hypothesis Testing

When you conduct a piece of quantitative research, you are inevitably attempting to answer a research question or hypothesis that you have set. One method of evaluating this research question is via a process called hypothesis testing , which is sometimes also referred to as significance testing . Since there are many facets to hypothesis testing, we start with the example we refer to throughout this guide.

An example of a lecturer's dilemma

Two statistics lecturers, Sarah and Mike, think that they use the best method to teach their students. Each lecturer has 50 statistics students who are studying a graduate degree in management. In Sarah's class, students have to attend one lecture and one seminar class every week, whilst in Mike's class students only have to attend one lecture. Sarah thinks that seminars, in addition to lectures, are an important teaching method in statistics, whilst Mike believes that lectures are sufficient by themselves and thinks that students are better off solving problems by themselves in their own time. This is the first year that Sarah has given seminars, but since they take up a lot of her time, she wants to make sure that she is not wasting her time and that seminars improve her students' performance.

The research hypothesis

The first step in hypothesis testing is to set a research hypothesis. In Sarah and Mike's study, the aim is to examine the effect that two different teaching methods – providing both lectures and seminar classes (Sarah), and providing lectures by themselves (Mike) – had on the performance of Sarah's 50 students and Mike's 50 students. More specifically, they want to determine whether performance is different between the two different teaching methods. Whilst Mike is skeptical about the effectiveness of seminars, Sarah clearly believes that giving seminars in addition to lectures helps her students do better than those in Mike's class. This leads to the following research hypothesis:

Before moving onto the second step of the hypothesis testing process, we need to take you on a brief detour to explain why you need to run hypothesis testing at all. This is explained next.

Sample to population

If you have measured individuals (or any other type of "object") in a study and want to understand differences (or any other type of effect), you can simply summarize the data you have collected. For example, if Sarah and Mike wanted to know which teaching method was the best, they could simply compare the performance achieved by the two groups of students – the group of students that took lectures and seminar classes, and the group of students that took lectures by themselves – and conclude that the best method was the teaching method which resulted in the highest performance. However, this is generally of only limited appeal because the conclusions could only apply to students in this study. However, if those students were representative of all statistics students on a graduate management degree, the study would have wider appeal.

In statistics terminology, the students in the study are the sample and the larger group they represent (i.e., all statistics students on a graduate management degree) is called the population . Given that the sample of statistics students in the study are representative of a larger population of statistics students, you can use hypothesis testing to understand whether any differences or effects discovered in the study exist in the population. In layman's terms, hypothesis testing is used to establish whether a research hypothesis extends beyond those individuals examined in a single study.

Another example could be taking a sample of 200 breast cancer sufferers in order to test a new drug that is designed to eradicate this type of cancer. As much as you are interested in helping these specific 200 cancer sufferers, your real goal is to establish that the drug works in the population (i.e., all breast cancer sufferers).

As such, by taking a hypothesis testing approach, Sarah and Mike want to generalize their results to a population rather than just the students in their sample. However, in order to use hypothesis testing, you need to re-state your research hypothesis as a null and alternative hypothesis. Before you can do this, it is best to consider the process/structure involved in hypothesis testing and what you are measuring. This structure is presented on the next page .

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What Is Hypothesis Testing?

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4 Step Process

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Hypothesis Testing: 4 Steps and Example

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

• Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
• The test provides evidence concerning the plausibility of the hypothesis, given the data.
• Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
• The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

• State the hypotheses.
• Formulate an analysis plan, which outlines how the data will be evaluated.
• Carry out the plan and analyze the sample data.
• Analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Example of Hypothesis Testing

If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

When Did Hypothesis Testing Begin?

Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

Sage. " Introduction to Hypothesis Testing ," Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."

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Hypothesis Testing - Analysis of Variance (ANOVA)

Lisa Sullivan, PhD

Professor of Biostatistics

Boston University School of Public Health

Introduction

This module will continue the discussion of hypothesis testing, where a specific statement or hypothesis is generated about a population parameter, and sample statistics are used to assess the likelihood that the hypothesis is true. The hypothesis is based on available information and the investigator's belief about the population parameters. The specific test considered here is called analysis of variance (ANOVA) and is a test of hypothesis that is appropriate to compare means of a continuous variable in two or more independent comparison groups. For example, in some clinical trials there are more than two comparison groups. In a clinical trial to evaluate a new medication for asthma, investigators might compare an experimental medication to a placebo and to a standard treatment (i.e., a medication currently being used). In an observational study such as the Framingham Heart Study, it might be of interest to compare mean blood pressure or mean cholesterol levels in persons who are underweight, normal weight, overweight and obese.  

The technique to test for a difference in more than two independent means is an extension of the two independent samples procedure discussed previously which applies when there are exactly two independent comparison groups. The ANOVA technique applies when there are two or more than two independent groups. The ANOVA procedure is used to compare the means of the comparison groups and is conducted using the same five step approach used in the scenarios discussed in previous sections. Because there are more than two groups, however, the computation of the test statistic is more involved. The test statistic must take into account the sample sizes, sample means and sample standard deviations in each of the comparison groups.

If one is examining the means observed among, say three groups, it might be tempting to perform three separate group to group comparisons, but this approach is incorrect because each of these comparisons fails to take into account the total data, and it increases the likelihood of incorrectly concluding that there are statistically significate differences, since each comparison adds to the probability of a type I error. Analysis of variance avoids these problemss by asking a more global question, i.e., whether there are significant differences among the groups, without addressing differences between any two groups in particular (although there are additional tests that can do this if the analysis of variance indicates that there are differences among the groups).

The fundamental strategy of ANOVA is to systematically examine variability within groups being compared and also examine variability among the groups being compared.

Learning Objectives

After completing this module, the student will be able to:

• Perform analysis of variance by hand
• Appropriately interpret results of analysis of variance tests
• Distinguish between one and two factor analysis of variance tests
• Identify the appropriate hypothesis testing procedure based on type of outcome variable and number of samples

The ANOVA Approach

Consider an example with four independent groups and a continuous outcome measure. The independent groups might be defined by a particular characteristic of the participants such as BMI (e.g., underweight, normal weight, overweight, obese) or by the investigator (e.g., randomizing participants to one of four competing treatments, call them A, B, C and D). Suppose that the outcome is systolic blood pressure, and we wish to test whether there is a statistically significant difference in mean systolic blood pressures among the four groups. The sample data are organized as follows:

The hypotheses of interest in an ANOVA are as follows:

• H 0 : μ 1 = μ 2 = μ 3 ... = μ k
• H 1 : Means are not all equal.

where k = the number of independent comparison groups.

In this example, the hypotheses are:

• H 0 : μ 1 = μ 2 = μ 3 = μ 4
• H 1 : The means are not all equal.

The null hypothesis in ANOVA is always that there is no difference in means. The research or alternative hypothesis is always that the means are not all equal and is usually written in words rather than in mathematical symbols. The research hypothesis captures any difference in means and includes, for example, the situation where all four means are unequal, where one is different from the other three, where two are different, and so on. The alternative hypothesis, as shown above, capture all possible situations other than equality of all means specified in the null hypothesis.

Test Statistic for ANOVA

The test statistic for testing H 0 : μ 1 = μ 2 = ... =   μ k is:

and the critical value is found in a table of probability values for the F distribution with (degrees of freedom) df 1 = k-1, df 2 =N-k. The table can be found in "Other Resources" on the left side of the pages.

NOTE: The test statistic F assumes equal variability in the k populations (i.e., the population variances are equal, or s 1 2 = s 2 2 = ... = s k 2 ). This means that the outcome is equally variable in each of the comparison populations. This assumption is the same as that assumed for appropriate use of the test statistic to test equality of two independent means. It is possible to assess the likelihood that the assumption of equal variances is true and the test can be conducted in most statistical computing packages. If the variability in the k comparison groups is not similar, then alternative techniques must be used.

The F statistic is computed by taking the ratio of what is called the "between treatment" variability to the "residual or error" variability. This is where the name of the procedure originates. In analysis of variance we are testing for a difference in means (H 0 : means are all equal versus H 1 : means are not all equal) by evaluating variability in the data. The numerator captures between treatment variability (i.e., differences among the sample means) and the denominator contains an estimate of the variability in the outcome. The test statistic is a measure that allows us to assess whether the differences among the sample means (numerator) are more than would be expected by chance if the null hypothesis is true. Recall in the two independent sample test, the test statistic was computed by taking the ratio of the difference in sample means (numerator) to the variability in the outcome (estimated by Sp).  

The decision rule for the F test in ANOVA is set up in a similar way to decision rules we established for t tests. The decision rule again depends on the level of significance and the degrees of freedom. The F statistic has two degrees of freedom. These are denoted df 1 and df 2 , and called the numerator and denominator degrees of freedom, respectively. The degrees of freedom are defined as follows:

df 1 = k-1 and df 2 =N-k,

where k is the number of comparison groups and N is the total number of observations in the analysis.   If the null hypothesis is true, the between treatment variation (numerator) will not exceed the residual or error variation (denominator) and the F statistic will small. If the null hypothesis is false, then the F statistic will be large. The rejection region for the F test is always in the upper (right-hand) tail of the distribution as shown below.

Rejection Region for F   Test with a =0.05, df 1 =3 and df 2 =36 (k=4, N=40)

For the scenario depicted here, the decision rule is: Reject H 0 if F > 2.87.

The ANOVA Procedure

We will next illustrate the ANOVA procedure using the five step approach. Because the computation of the test statistic is involved, the computations are often organized in an ANOVA table. The ANOVA table breaks down the components of variation in the data into variation between treatments and error or residual variation. Statistical computing packages also produce ANOVA tables as part of their standard output for ANOVA, and the ANOVA table is set up as follows: 

where  

• X = individual observation,
• k = the number of treatments or independent comparison groups, and
• N = total number of observations or total sample size.

The ANOVA table above is organized as follows.

• The first column is entitled "Source of Variation" and delineates the between treatment and error or residual variation. The total variation is the sum of the between treatment and error variation.
• The second column is entitled "Sums of Squares (SS)" . The between treatment sums of squares is

and is computed by summing the squared differences between each treatment (or group) mean and the overall mean. The squared differences are weighted by the sample sizes per group (n j ). The error sums of squares is:

and is computed by summing the squared differences between each observation and its group mean (i.e., the squared differences between each observation in group 1 and the group 1 mean, the squared differences between each observation in group 2 and the group 2 mean, and so on). The double summation ( SS ) indicates summation of the squared differences within each treatment and then summation of these totals across treatments to produce a single value. (This will be illustrated in the following examples). The total sums of squares is:

and is computed by summing the squared differences between each observation and the overall sample mean. In an ANOVA, data are organized by comparison or treatment groups. If all of the data were pooled into a single sample, SST would reflect the numerator of the sample variance computed on the pooled or total sample. SST does not figure into the F statistic directly. However, SST = SSB + SSE, thus if two sums of squares are known, the third can be computed from the other two.

• The third column contains degrees of freedom . The between treatment degrees of freedom is df 1 = k-1. The error degrees of freedom is df 2 = N - k. The total degrees of freedom is N-1 (and it is also true that (k-1) + (N-k) = N-1).
• The fourth column contains "Mean Squares (MS)" which are computed by dividing sums of squares (SS) by degrees of freedom (df), row by row. Specifically, MSB=SSB/(k-1) and MSE=SSE/(N-k). Dividing SST/(N-1) produces the variance of the total sample. The F statistic is in the rightmost column of the ANOVA table and is computed by taking the ratio of MSB/MSE.  

A clinical trial is run to compare weight loss programs and participants are randomly assigned to one of the comparison programs and are counseled on the details of the assigned program. Participants follow the assigned program for 8 weeks. The outcome of interest is weight loss, defined as the difference in weight measured at the start of the study (baseline) and weight measured at the end of the study (8 weeks), measured in pounds.  

Three popular weight loss programs are considered. The first is a low calorie diet. The second is a low fat diet and the third is a low carbohydrate diet. For comparison purposes, a fourth group is considered as a control group. Participants in the fourth group are told that they are participating in a study of healthy behaviors with weight loss only one component of interest. The control group is included here to assess the placebo effect (i.e., weight loss due to simply participating in the study). A total of twenty patients agree to participate in the study and are randomly assigned to one of the four diet groups. Weights are measured at baseline and patients are counseled on the proper implementation of the assigned diet (with the exception of the control group). After 8 weeks, each patient's weight is again measured and the difference in weights is computed by subtracting the 8 week weight from the baseline weight. Positive differences indicate weight losses and negative differences indicate weight gains. For interpretation purposes, we refer to the differences in weights as weight losses and the observed weight losses are shown below.

Is there a statistically significant difference in the mean weight loss among the four diets?  We will run the ANOVA using the five-step approach.

• Step 1. Set up hypotheses and determine level of significance

H 0 : μ 1 = μ 2 = μ 3 = μ 4 H 1 : Means are not all equal              α=0.05

• Step 2. Select the appropriate test statistic.  

The test statistic is the F statistic for ANOVA, F=MSB/MSE.

• Step 3. Set up decision rule.  

The appropriate critical value can be found in a table of probabilities for the F distribution(see "Other Resources"). In order to determine the critical value of F we need degrees of freedom, df 1 =k-1 and df 2 =N-k. In this example, df 1 =k-1=4-1=3 and df 2 =N-k=20-4=16. The critical value is 3.24 and the decision rule is as follows: Reject H 0 if F > 3.24.

• Step 4. Compute the test statistic.  

To organize our computations we complete the ANOVA table. In order to compute the sums of squares we must first compute the sample means for each group and the overall mean based on the total sample.  

We can now compute

So, in this case:

Next we compute,

SSE requires computing the squared differences between each observation and its group mean. We will compute SSE in parts. For the participants in the low calorie diet:  

For the participants in the low fat diet:  

For the participants in the low carbohydrate diet:  

For the participants in the control group:

We can now construct the ANOVA table .

• Step 5. Conclusion.  

We reject H 0 because 8.43 > 3.24. We have statistically significant evidence at α=0.05 to show that there is a difference in mean weight loss among the four diets.    

ANOVA is a test that provides a global assessment of a statistical difference in more than two independent means. In this example, we find that there is a statistically significant difference in mean weight loss among the four diets considered. In addition to reporting the results of the statistical test of hypothesis (i.e., that there is a statistically significant difference in mean weight losses at α=0.05), investigators should also report the observed sample means to facilitate interpretation of the results. In this example, participants in the low calorie diet lost an average of 6.6 pounds over 8 weeks, as compared to 3.0 and 3.4 pounds in the low fat and low carbohydrate groups, respectively. Participants in the control group lost an average of 1.2 pounds which could be called the placebo effect because these participants were not participating in an active arm of the trial specifically targeted for weight loss. Are the observed weight losses clinically meaningful?

Another ANOVA Example

Calcium is an essential mineral that regulates the heart, is important for blood clotting and for building healthy bones. The National Osteoporosis Foundation recommends a daily calcium intake of 1000-1200 mg/day for adult men and women. While calcium is contained in some foods, most adults do not get enough calcium in their diets and take supplements. Unfortunately some of the supplements have side effects such as gastric distress, making them difficult for some patients to take on a regular basis.  

 A study is designed to test whether there is a difference in mean daily calcium intake in adults with normal bone density, adults with osteopenia (a low bone density which may lead to osteoporosis) and adults with osteoporosis. Adults 60 years of age with normal bone density, osteopenia and osteoporosis are selected at random from hospital records and invited to participate in the study. Each participant's daily calcium intake is measured based on reported food intake and supplements. The data are shown below.   

Is there a statistically significant difference in mean calcium intake in patients with normal bone density as compared to patients with osteopenia and osteoporosis? We will run the ANOVA using the five-step approach.

H 0 : μ 1 = μ 2 = μ 3 H 1 : Means are not all equal                            α=0.05

In order to determine the critical value of F we need degrees of freedom, df 1 =k-1 and df 2 =N-k.   In this example, df 1 =k-1=3-1=2 and df 2 =N-k=18-3=15. The critical value is 3.68 and the decision rule is as follows: Reject H 0 if F > 3.68.

To organize our computations we will complete the ANOVA table. In order to compute the sums of squares we must first compute the sample means for each group and the overall mean.  

 If we pool all N=18 observations, the overall mean is 817.8.

We can now compute:

Substituting:

SSE requires computing the squared differences between each observation and its group mean. We will compute SSE in parts. For the participants with normal bone density:

For participants with osteopenia:

For participants with osteoporosis:

We do not reject H 0 because 1.395 < 3.68. We do not have statistically significant evidence at a =0.05 to show that there is a difference in mean calcium intake in patients with normal bone density as compared to osteopenia and osterporosis. Are the differences in mean calcium intake clinically meaningful? If so, what might account for the lack of statistical significance?

One-Way ANOVA in R

The video below by Mike Marin demonstrates how to perform analysis of variance in R. It also covers some other statistical issues, but the initial part of the video will be useful to you.

Two-Factor ANOVA

The ANOVA tests described above are called one-factor ANOVAs. There is one treatment or grouping factor with k > 2 levels and we wish to compare the means across the different categories of this factor. The factor might represent different diets, different classifications of risk for disease (e.g., osteoporosis), different medical treatments, different age groups, or different racial/ethnic groups. There are situations where it may be of interest to compare means of a continuous outcome across two or more factors. For example, suppose a clinical trial is designed to compare five different treatments for joint pain in patients with osteoarthritis. Investigators might also hypothesize that there are differences in the outcome by sex. This is an example of a two-factor ANOVA where the factors are treatment (with 5 levels) and sex (with 2 levels). In the two-factor ANOVA, investigators can assess whether there are differences in means due to the treatment, by sex or whether there is a difference in outcomes by the combination or interaction of treatment and sex. Higher order ANOVAs are conducted in the same way as one-factor ANOVAs presented here and the computations are again organized in ANOVA tables with more rows to distinguish the different sources of variation (e.g., between treatments, between men and women). The following example illustrates the approach.

Consider the clinical trial outlined above in which three competing treatments for joint pain are compared in terms of their mean time to pain relief in patients with osteoarthritis. Because investigators hypothesize that there may be a difference in time to pain relief in men versus women, they randomly assign 15 participating men to one of the three competing treatments and randomly assign 15 participating women to one of the three competing treatments (i.e., stratified randomization). Participating men and women do not know to which treatment they are assigned. They are instructed to take the assigned medication when they experience joint pain and to record the time, in minutes, until the pain subsides. The data (times to pain relief) are shown below and are organized by the assigned treatment and sex of the participant.

Table of Time to Pain Relief by Treatment and Sex

The analysis in two-factor ANOVA is similar to that illustrated above for one-factor ANOVA. The computations are again organized in an ANOVA table, but the total variation is partitioned into that due to the main effect of treatment, the main effect of sex and the interaction effect. The results of the analysis are shown below (and were generated with a statistical computing package - here we focus on interpretation). 

 ANOVA Table for Two-Factor ANOVA

There are 4 statistical tests in the ANOVA table above. The first test is an overall test to assess whether there is a difference among the 6 cell means (cells are defined by treatment and sex). The F statistic is 20.7 and is highly statistically significant with p=0.0001. When the overall test is significant, focus then turns to the factors that may be driving the significance (in this example, treatment, sex or the interaction between the two). The next three statistical tests assess the significance of the main effect of treatment, the main effect of sex and the interaction effect. In this example, there is a highly significant main effect of treatment (p=0.0001) and a highly significant main effect of sex (p=0.0001). The interaction between the two does not reach statistical significance (p=0.91). The table below contains the mean times to pain relief in each of the treatments for men and women (Note that each sample mean is computed on the 5 observations measured under that experimental condition).  

Mean Time to Pain Relief by Treatment and Gender

Treatment A appears to be the most efficacious treatment for both men and women. The mean times to relief are lower in Treatment A for both men and women and highest in Treatment C for both men and women. Across all treatments, women report longer times to pain relief (See below).  

Notice that there is the same pattern of time to pain relief across treatments in both men and women (treatment effect). There is also a sex effect - specifically, time to pain relief is longer in women in every treatment.  

Suppose that the same clinical trial is replicated in a second clinical site and the following data are observed.

Table - Time to Pain Relief by Treatment and Sex - Clinical Site 2

The ANOVA table for the data measured in clinical site 2 is shown below.

Table - Summary of Two-Factor ANOVA - Clinical Site 2

Notice that the overall test is significant (F=19.4, p=0.0001), there is a significant treatment effect, sex effect and a highly significant interaction effect. The table below contains the mean times to relief in each of the treatments for men and women.  

Table - Mean Time to Pain Relief by Treatment and Gender - Clinical Site 2

Notice that now the differences in mean time to pain relief among the treatments depend on sex. Among men, the mean time to pain relief is highest in Treatment A and lowest in Treatment C. Among women, the reverse is true. This is an interaction effect (see below).  

Notice above that the treatment effect varies depending on sex. Thus, we cannot summarize an overall treatment effect (in men, treatment C is best, in women, treatment A is best).    

When interaction effects are present, some investigators do not examine main effects (i.e., do not test for treatment effect because the effect of treatment depends on sex). This issue is complex and is discussed in more detail in a later module. 

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Statistics By Jim

Making statistics intuitive

Types I & Type II Errors in Hypothesis Testing

By Jim Frost 8 Comments

In hypothesis testing, a Type I error is a false positive while a Type II error is a false negative. In this blog post, you will learn about these two types of errors, their causes, and how to manage them.

Hypothesis tests use sample data to make inferences about the properties of a population . You gain tremendous benefits by working with random samples because it is usually impossible to measure the entire population.

However, there are tradeoffs when you use samples. The samples we use are typically a minuscule percentage of the entire population. Consequently, they occasionally misrepresent the population severely enough to cause hypothesis tests to make Type I and Type II errors.

Potential Outcomes in Hypothesis Testing

Hypothesis testing  is a procedure in inferential statistics that assesses two mutually exclusive theories about the properties of a population. For a generic hypothesis test, the two hypotheses are as follows:

• Null hypothesis : There is no effect
• Alternative hypothesis : There is an effect.

The sample data must provide sufficient evidence to reject the null hypothesis and conclude that the effect exists in the population. Ideally, a hypothesis test fails to reject the null hypothesis when the effect is not present in the population, and it rejects the null hypothesis when the effect exists.

Statisticians define two types of errors in hypothesis testing. Creatively, they call these errors Type I and Type II errors. Both types of error relate to incorrect conclusions about the null hypothesis.

The table summarizes the four possible outcomes for a hypothesis test.

Related post : How Hypothesis Tests Work: P-values and the Significance Level

Fire alarm analogy for the types of errors

Using hypothesis tests correctly improves your chances of drawing trustworthy conclusions. However, errors are bound to occur.

Unlike the fire alarm analogy, there is no sure way to determine whether an error occurred after you perform a hypothesis test. Typically, a clearer picture develops over time as other researchers conduct similar studies and an overall pattern of results appears. Seeing how your results fit in with similar studies is a crucial step in assessing your study’s findings.

Now, let’s take a look at each type of error in more depth.

Type I Error: False Positives

When you see a p-value that is less than your significance level , you get excited because your results are statistically significant. However, it could be a type I error . The supposed effect might not exist in the population. Again, there is usually no warning when this occurs.

Why do these errors occur? It comes down to sample error. Your random sample has overestimated the effect by chance. It was the luck of the draw. This type of error doesn’t indicate that the researchers did anything wrong. The experimental design, data collection, data validity , and statistical analysis can all be correct, and yet this type of error still occurs.

Even though we don’t know for sure which studies have false positive results, we do know their rate of occurrence. The rate of occurrence for Type I errors equals the significance level of the hypothesis test, which is also known as alpha (α).

The significance level is an evidentiary standard that you set to determine whether your sample data are strong enough to reject the null hypothesis. Hypothesis tests define that standard using the probability of rejecting a null hypothesis that is actually true. You set this value based on your willingness to risk a false positive.

Related post : How to Interpret P-values Correctly

Using the significance level to set the Type I error rate

When the significance level is 0.05 and the null hypothesis is true, there is a 5% chance that the test will reject the null hypothesis incorrectly. If you set alpha to 0.01, there is a 1% of a false positive. If 5% is good, then 1% seems even better, right? As you’ll see, there is a tradeoff between Type I and Type II errors. If you hold everything else constant, as you reduce the chance for a false positive, you increase the opportunity for a false negative.

Type I errors are relatively straightforward. The math is beyond the scope of this article, but statisticians designed hypothesis tests to incorporate everything that affects this error rate so that you can specify it for your studies. As long as your experimental design is sound, you collect valid data, and the data satisfy the assumptions of the hypothesis test, the Type I error rate equals the significance level that you specify. However, if there is a problem in one of those areas, it can affect the false positive rate.

Warning about a potential misinterpretation of Type I errors and the Significance Level

When the null hypothesis is correct for the population, the probability that a test produces a false positive equals the significance level. However, when you look at a statistically significant test result, you cannot state that there is a 5% chance that it represents a false positive.

Why is that the case? Imagine that we perform 100 studies on a population where the null hypothesis is true. If we use a significance level of 0.05, we’d expect that five of the studies will produce statistically significant results—false positives. Afterward, when we go to look at those significant studies, what is the probability that each one is a false positive? Not 5 percent but 100%!

That scenario also illustrates a point that I made earlier. The true picture becomes more evident after repeated experimentation. Given the pattern of results that are predominantly not significant, it is unlikely that an effect exists in the population.

Type II Error: False Negatives

When you perform a hypothesis test and your p-value is greater than your significance level, your results are not statistically significant. That’s disappointing because your sample provides insufficient evidence for concluding that the effect you’re studying exists in the population. However, there is a chance that the effect is present in the population even though the test results don’t support it. If that’s the case, you’ve just experienced a Type II error . The probability of making a Type II error is known as beta (β).

What causes Type II errors? Whereas Type I errors are caused by one thing, sample error, there are a host of possible reasons for Type II errors—small effect sizes, small sample sizes, and high data variability. Furthermore, unlike Type I errors, you can’t set the Type II error rate for your analysis. Instead, the best that you can do is estimate it before you begin your study by approximating properties of the alternative hypothesis that you’re studying. When you do this type of estimation, it’s called power analysis.

To estimate the Type II error rate, you create a hypothetical probability distribution that represents the properties of a true alternative hypothesis. However, when you’re performing a hypothesis test, you typically don’t know which hypothesis is true, much less the specific properties of the distribution for the alternative hypothesis. Consequently, the true Type II error rate is usually unknown!

Type II errors and the power of the analysis

The Type II error rate (beta) is the probability of a false negative. Therefore, the inverse of Type II errors is the probability of correctly detecting an effect. Statisticians refer to this concept as the power of a hypothesis test. Consequently, 1 – β = the statistical power. Analysts typically estimate power rather than beta directly.

If you read my post about power and sample size analysis , you know that the three factors that affect power are sample size, variability in the population, and the effect size. As you design your experiment, you can enter estimates of these three factors into statistical software and it calculates the estimated power for your test.

Suppose you perform a power analysis for an upcoming study and calculate an estimated power of 90%. For this study, the estimated Type II error rate is 10% (1 – 0.9). Keep in mind that variability and effect size are based on estimates and guesses. Consequently, power and the Type II error rate are just estimates rather than something you set directly. These estimates are only as good as the inputs into your power analysis.

Low variability and larger effect sizes decrease the Type II error rate, which increases the statistical power. However, researchers usually have less control over those aspects of a hypothesis test. Typically, researchers have the most control over sample size, making it the critical way to manage your Type II error rate. Holding everything else constant, increasing the sample size reduces the Type II error rate and increases power.

Learn more about Power in Statistics .

Graphing Type I and Type II Errors

The graph below illustrates the two types of errors using two sampling distributions. The critical region line represents the point at which you reject or fail to reject the null hypothesis. Of course, when you perform the hypothesis test, you don’t know which hypothesis is correct. And, the properties of the distribution for the alternative hypothesis are usually unknown. However, use this graph to understand the general nature of these errors and how they are related.

The distribution on the left represents the null hypothesis. If the null hypothesis is true, you only need to worry about Type I errors, which is the shaded portion of the null hypothesis distribution. The rest of the null distribution represents the correct decision of failing to reject the null.

On the other hand, if the alternative hypothesis is true, you need to worry about Type II errors. The shaded region on the alternative hypothesis distribution represents the Type II error rate. The rest of the alternative distribution represents the probability of correctly detecting an effect—power.

Moving the critical value line is equivalent to changing the significance level. If you move the line to the left, you’re increasing the significance level (e.g., α 0.05 to 0.10). Holding everything else constant, this adjustment increases the Type I error rate while reducing the Type II error rate. Moving the line to the right reduces the significance level (e.g., α 0.05 to 0.01), which decreases the Type I error rate but increases the type II error rate.

Is One Error Worse Than the Other?

As you’ve seen, the nature of the two types of error, their causes, and the certainty of their rates of occurrence are all very different.

A common question is whether one type of error is worse than the other? Statisticians designed hypothesis tests to control Type I errors while Type II errors are much less defined. Consequently, many statisticians state that it is better to fail to detect an effect when it exists than it is to conclude an effect exists when it doesn’t. That is to say, there is a tendency to assume that Type I errors are worse.

However, reality is more complex than that. You should carefully consider the consequences of each type of error for your specific test.

Suppose you are assessing the strength of a new jet engine part that is under consideration. Peoples lives are riding on the part’s strength. A false negative in this scenario merely means that the part is strong enough but the test fails to detect it. This situation does not put anyone’s life at risk. On the other hand, Type I errors are worse in this situation because they indicate the part is strong enough when it is not.

Now suppose that the jet engine part is already in use but there are concerns about it failing. In this case, you want the test to be more sensitive to detecting problems even at the risk of false positives. Type II errors are worse in this scenario because the test fails to recognize the problem and leaves these problematic parts in use for longer.

Using hypothesis tests effectively requires that you understand their error rates. By setting the significance level and estimating your test’s power, you can manage both error rates so they meet your requirements.

The error rates in this post are all for individual tests. If you need to perform multiple comparisons, such as comparing group means in ANOVA, you’ll need to use post hoc tests to control the experiment-wise error rate  or use the Bonferroni correction .

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Reader Interactions

June 4, 2024 at 2:04 pm

Very informative.

June 9, 2023 at 9:54 am

Hi Jim- I just signed up for your newsletter and this is my first question to you. I am not a statistician but work with them in my professional life as a QC consultant in biopharmaceutical development. I have a question about Type I and Type II errors in the realm of equivalence testing using two one sided difference testing (TOST). In a recent 2020 publication that I co-authored with a statistician, we stated that the probability of concluding non-equivalence when that is the truth, (which is the opposite of power, the probability of concluding equivalence when it is correct) is 1-2*alpha. This made sense to me because one uses a 90% confidence interval on a mean to evaluate whether the result is within established equivalence bounds with an alpha set to 0.05. However, it appears that specificity (1-alpha) is always the case as is power always being 1-beta. For equivalence testing the latter is 1-2*beta/2 but for specificity it stays as 1-alpha because only one of the null hypotheses in a two-sided test can fail at one time. I still see 1-2*alpha as making more sense as we show in Figure 3 of our paper which shows the white space under the distribution of the alternative hypothesis as 1-2 alpha. The paper can be downloaded as open access here if that would make my question more clear. https://bioprocessingjournal.com/index.php/article-downloads/890-vol-19-open-access-2020-defining-therapeutic-window-for-viral-vectors-a-statistical-framework-to-improve-consistency-in-assigning-product-dose-values I have consulted with other statistical colleagues and cannot get consensus so I would love your opinion and explanation! Thanks in advance!

June 10, 2023 at 1:00 am

Let me preface my response by saying that I’m not an expert in equivalence testing. But here’s my best guess about your question.

The alpha is for each of the hypothesis tests. Each one has a type I error rate of 0.05. Or, as you say, a specificity of 1-alpha. However, there are two tests so we need to consider the family-wise error rate. The formula is the following:

FWER = 1 – (1 – α)^N

Where N is the number of hypothesis tests.

For two tests, there’s a family-wise error rate of 0.0975. Or a family-wise specificity of 0.9025.

However, I believe they use 90% CI for a different reason (although it’s a very close match to the family-wise error rate). The 90% CI provides consistent results with the two one-side 95% tests. In other words, if the 90% CI is within the equivalency bounds, then the two tests will be significant. If the CI extends above the upper bound, the corresponding test won’t be significant. Etc.

However, using either rational, I’d say the overall type I error rate is about 0.1.

I hope that answers your question. And, again, I’m not an expert in this particular test.

July 18, 2022 at 5:15 am

Thank you for your valuable content. I have a question regarding correcting for multiple tests. My question is: for exactly how many tests should I correct in the scenario below?

Background: I’m testing for differences between groups A (patient group) and B (control group) in variable X. Variable X is a biological variable present in the body’s left and right side. Variable Y is a questionnaire for group A.

Step 1. Is there a significant difference within groups in the weight of left and right variable X? (I will conduct two paired sample t-tests)


If I find a significant difference in step 1, then I will conduct steps 2A and 2B. However, if I don’t find a significant difference in step 1, then I will only conduct step 2C.

Step 2A. Is there a significant difference between groups in left variable X? (I will conduct one independent sample t-test) Step 2B. Is there a significant difference between groups in right variable X? (I will conduct one independent sample t-test)

Step 2C. Is there a significant difference between groups in total variable X (left + right variable X)? (I will conduct one independent sample t-test)

If I find a significant difference in step 1, then I will conduct with steps 3A and 3B. However, if I don’t find a significant difference in step 1, then I will only conduct step 3C.

Step 3A. Is there a significant correlation between left variable X in group A and variable Y? (I will conduct Pearson correlation) Step 3B. Is there a significant correlation between right variable X in group A and variable Y? (I will conduct Pearson correlation)

Step 3C. Is there a significant correlation between total variable X in group A and variable Y? (I will conduct a Pearson correlation)

Regards, De

January 2, 2021 at 1:57 pm

I should say that being a budding statistician, this site seems to be pretty reliable. I have few doubts in here. It would be great if you can clarify it:

“A significance level of 0.05 indicates a 5% risk of concluding that a difference exists when there is no actual difference. ”

My understanding : When we say that the significance level is 0.05 then it means we are taking 5% risk to support alternate hypothesis even though there is no difference ?( I think i am not allowed to say Null is true, because null is assumed to be true/ Right)

January 2, 2021 at 6:48 pm

The sentence as I write it is correct. Here’s a simple way to understand it. Imagine you’re conducting a computer simulation where you control the population parameters and have the computer draw random samples from the populations that you define. Now, imagine you draw samples from two populations where the means and standard deviations are equal. You know this for a fact because you set the parameters yourself. Then you conduct a series of 2-sample t-tests.

In this example, you know the null hypothesis is correct. However, thanks to random sampling error, some proportion of the t-tests will have statistically significant results (i.e., false positives or Type I errors). The proportion of false positives will equal your significance level over the long run.

Of course, in real-world experiments, you never know for sure whether the null is true or not. However, given the properties of the hypothesis, you do know what proportion of tests will give you a false positive IF the null is true–and that’s the significance level.

I’m thinking through the wording of how you wrote it and I believe it is equivalent to what I wrote. If there is no difference (the null is true), then you have a 5% chance of incorrectly supporting the alternative. And, again, you’re correct that in the real world you don’t know for sure whether the null is true. But, you can still know the false positive (Type I) error rate. For more information about that property, read my post about how hypothesis tests work .

July 9, 2018 at 11:43 am

I like to use the analogy of a trial. The null hypothesis is that the defendant is innocent. A type I error would be convicting an innocent person and a type II error would be acquitting a guilty one. I like to think that our system makes a type I error very unlikely with the trade off being that a type II error is greater.

July 9, 2018 at 12:03 pm

Hi Doug, I think that is an excellent analogy on multiple levels. As you mention, a trial would set a high bar for the significance level by choosing a very low value for alpha. This helps prevent innocent people from being convicted (Type I error) but does increase the probability of allowing the guilty to go free (Type II error). I often refer to the significant level as a evidentiary standard with this legalistic analogy in mind.

Additionally, in the justice system in the U.S., there is a presumption of innocence and the prosecutor must present sufficient evidence to prove that the defendant is guilty. That’s just like in a hypothesis test where the assumption is that the null hypothesis is true and your sample must contain sufficient evidence to be able to reject the null hypothesis and suggest that the effect exists in the population.

This analogy even works for the similarities behind the phrases “Not guilty” and “Fail to reject the null hypothesis.” In both cases, you aren’t proving innocence or that the null hypothesis is true. When a defendant is “not guilty” it might be that the evidence was insufficient to convince the jury. In a hypothesis test, when you fail to reject the null hypothesis, it’s possible that an effect exists in the population but you have insufficient evidence to detect it. Perhaps the effect exists but the sample size or effect size is too small, or the variability might be too high.

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How to Write a Hypothesis

If I [do something], then [this] will happen.

This basic statement/formula should be pretty familiar to all of you as it is the starting point of almost every scientific project or paper. It is a hypothesis – a statement that showcases what you “think” will happen during an experiment. This assumption is made based on the knowledge, facts, and data you already have.

How do you write a hypothesis? If you have a clear understanding of the proper structure of a hypothesis, you should not find it too hard to create one. However, if you have never written a hypothesis before, you might find it a bit frustrating. In this article from EssayPro - custom essay writing services , we are going to tell you everything you need to know about hypotheses, their types, and practical tips for writing them.

Hypothesis Definition

According to the definition, a hypothesis is an assumption one makes based on existing knowledge. To elaborate, it is a statement that translates the initial research question into a logical prediction shaped on the basis of available facts and evidence. To solve a specific problem, one first needs to identify the research problem (research question), conduct initial research, and set out to answer the given question by performing experiments and observing their outcomes. However, before one can move to the experimental part of the research, they should first identify what they expect to see for results. At this stage, a scientist makes an educated guess and writes a hypothesis that he or she is going to prove or refute in the course of their study.

Get Help With Writing a Hypothesis Now!

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A hypothesis can also be seen as a form of development of knowledge. It is a well-grounded assumption put forward to clarify the properties and causes of the phenomena being studied.

As a rule, a hypothesis is formed based on a number of observations and examples that confirm it. This way, it looks plausible as it is backed up with some known information. The hypothesis is subsequently proved by turning it into an established fact or refuted (for example, by pointing out a counterexample), which allows it to attribute it to the category of false statements.

As a student, you may be asked to create a hypothesis statement as a part of your academic papers. Hypothesis-based approaches are commonly used among scientific academic works, including but not limited to research papers, theses, and dissertations.

Note that in some disciplines, a hypothesis statement is called a thesis statement. However, its essence and purpose remain unchanged – this statement aims to make an assumption regarding the outcomes of the investigation that will either be proved or refuted.

Characteristics and Sources of a Hypothesis

Now, as you know what a hypothesis is in a nutshell, let’s look at the key characteristics that define it:

• It has to be clear and accurate in order to look reliable.
• It has to be specific.
• There should be scope for further investigation and experiments.
• A hypothesis should be explained in simple language—while retaining its significance.
• If you are making a relational hypothesis, two essential elements you have to include are variables and the relationship between them.

The main sources of a hypothesis are:

• Scientific theories.
• Observations from previous studies and current experiences.
• The resemblance among different phenomena.
• General patterns that affect people’s thinking process.

Types of Hypothesis

Basically, there are two major types of scientific hypothesis: alternative and null.

• Alternative Hypothesis

This type of hypothesis is generally denoted as H1. This statement is used to identify the expected outcome of your research. According to the alternative hypothesis definition, this type of hypothesis can be further divided into two subcategories:

• Directional — a statement that explains the direction of the expected outcomes. Sometimes this type of hypothesis is used to study the relationship between variables rather than comparing between the groups.
• Non-directional — unlike the directional alternative hypothesis, a non-directional one does not imply a specific direction of the expected outcomes.

Now, let’s see an alternative hypothesis example for each type:

Directional: Attending more lectures will result in improved test scores among students. Non-directional: Lecture attendance will influence test scores among students.

Notice how in the directional hypothesis we specified that the attendance of more lectures will boost student’s performance on tests, whereas in the non-directional hypothesis we only stated that there is a relationship between the two variables (i.e. lecture attendance and students’ test scores) but did not specify whether the performance will improve or decrease.

• Null Hypothesis

This type of hypothesis is generally denoted as H0. This statement is the complete opposite of what you expect or predict will happen throughout the course of your study—meaning it is the opposite of your alternative hypothesis. Simply put, a null hypothesis claims that there is no exact or actual correlation between the variables defined in the hypothesis.

To give you a better idea of how to write a null hypothesis, here is a clear example: Lecture attendance has no effect on student’s test scores.

Both of these types of hypotheses provide specific clarifications and restatements of the research problem. The main difference between these hypotheses and a research problem is that the latter is just a question that can’t be tested, whereas hypotheses can.

Based on the alternative and null hypothesis examples provided earlier, we can conclude that the importance and main purpose of these hypotheses are that they deliver a rough description of the subject matter. The main purpose of these statements is to give an investigator a specific guess that can be directly tested in a study. Simply put, a hypothesis outlines the framework, scope, and direction for the study. Although null and alternative hypotheses are the major types, there are also a few more to keep in mind:

Research Hypothesis — a statement that is used to test the correlation between two or more variables.

For example: Eating vitamin-rich foods affects human health.

Simple Hypothesis — a statement used to indicate the correlation between one independent and one dependent variable.

For example: Eating more vegetables leads to better immunity.

Complex Hypothesis — a statement used to indicate the correlation between two or more independent variables and two or more dependent variables.

For example: Eating more fruits and vegetables leads to better immunity, weight loss, and lower risk of diseases.

Associative and Causal Hypothesis — an associative hypothesis is a statement used to indicate the correlation between variables under the scenario when a change in one variable inevitably changes the other variable. A causal hypothesis is a statement that highlights the cause and effect relationship between variables.

Be sure to read how to write a DBQ - this article will expand your understanding.

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Hypothesis vs Prediction

When speaking of hypotheses, another term that comes to mind is prediction. These two terms are often used interchangeably, which can be rather confusing. Although both a hypothesis and prediction can generally be defined as “guesses” and can be easy to confuse, these terms are different. The main difference between a hypothesis and a prediction is that the first is predominantly used in science, while the latter is most often used outside of science.

Simply put, a hypothesis is an intelligent assumption. It is a guess made regarding the nature of the unknown (or less known) phenomena based on existing knowledge, studies, and/or series of experiments, and is otherwise grounded by valid facts. The main purpose of a hypothesis is to use available facts to create a logical relationship between variables in order to provide a more precise scientific explanation. Additionally, hypotheses are statements that can be tested with further experiments. It is an assumption you make regarding the flow and outcome(s) of your research study.

A prediction, on the contrary, is a guess that often lacks grounding. Although, in theory, a prediction can be scientific, in most cases it is rather fictional—i.e. a pure guess that is not based on current knowledge and/or facts. As a rule, predictions are linked to foretelling events that may or may not occur in the future. Often, a person who makes predictions has little or no actual knowledge of the subject matter he or she makes the assumption about.

Another big difference between these terms is in the methodology used to prove each of them. A prediction can only be proven once. You can determine whether it is right or wrong only upon the occurrence or non-occurrence of the predicted event. A hypothesis, on the other hand, offers scope for further testing and experiments. Additionally, a hypothesis can be proven in multiple stages. This basically means that a single hypothesis can be proven or refuted numerous times by different scientists who use different scientific tools and methods.

To give you a better idea of how a hypothesis is different from a prediction, let’s look at the following examples:

Hypothesis: If I eat more vegetables and fruits, then I will lose weight faster.

This is a hypothesis because it is based on generally available knowledge (i.e. fruits and vegetables include fewer calories compared to other foods) and past experiences (i.e. people who give preference to healthier foods like fruits and vegetables are losing weight easier). It is still a guess, but it is based on facts and can be tested with an experiment.

Prediction: The end of the world will occur in 2023.

This is a prediction because it foretells future events. However, this assumption is fictional as it doesn’t have any actual grounded evidence supported by facts.

Based on everything that was said earlier and our examples, we can highlight the following key takeaways:

• A hypothesis, unlike a prediction, is a more intelligent assumption based on facts.
• Hypotheses define existing variables and analyze the relationship(s) between them.
• Predictions are most often fictional and lack grounding.
• A prediction is most often used to foretell events in the future.
• A prediction can only be proven once – when the predicted event occurs or doesn’t occur. 
• A hypothesis can remain a hypothesis even if one scientist has already proven or disproven it. Other scientists in the future can obtain a different result using other methods and tools.

We also recommend that you read about some informative essay topics .

Now, as you know what a hypothesis is, what types of it exist, and how it differs from a prediction, you are probably wondering how to state a hypothesis. In this section, we will guide you through the main stages of writing a good hypothesis and provide handy tips and examples to help you overcome this challenge:

1. Define Your Research Question

Here is one thing to keep in mind – regardless of the paper or project you are working on, the process should always start with asking the right research question. A perfect research question should be specific, clear, focused (meaning not too broad), and manageable.

Example: How does eating fruits and vegetables affect human health?

2. Conduct Your Basic Initial Research

As you already know, a hypothesis is an educated guess of the expected results and outcomes of an investigation. Thus, it is vital to collect some information before you can make this assumption.

At this stage, you should find an answer to your research question based on what has already been discovered. Search for facts, past studies, theories, etc. Based on the collected information, you should be able to make a logical and intelligent guess.

3. Formulate a Hypothesis

Based on the initial research, you should have a certain idea of what you may find throughout the course of your research. Use this knowledge to shape a clear and concise hypothesis.

Based on the type of project you are working on, and the type of hypothesis you are planning to use, you can restate your hypothesis in several different ways:

Non-directional: Eating fruits and vegetables will affect one’s human physical health. Directional: Eating fruits and vegetables will positively affect one’s human physical health. Null: Eating fruits and vegetables will have no effect on one’s human physical health.

4. Refine Your Hypothesis

Finally, the last stage of creating a good hypothesis is refining what you’ve got. During this step, you need to define whether your hypothesis:

• Has clear and relevant variables;
• Identifies the relationship between its variables;
• Is specific and testable;
• Suggests a predicted result of the investigation or experiment.

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Hypothesis Examples

Following a step-by-step guide and tips from our essay writers for hire , you should be able to create good hypotheses with ease. To give you a starting point, we have also compiled a list of different research questions with one hypothesis and one null hypothesis example for each:

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Sometimes, coping with a large academic load is just too much for a student to handle. Papers like research papers and dissertations can take too much time and effort to write, and, often, a hypothesis is a necessary starting point to get the task on track. Writing or editing a hypothesis is not as easy as it may seem. However, if you need help with forming it, the team at EssayPro is always ready to come to your rescue! If you’re feeling stuck, or don’t have enough time to cope with other tasks, don’t hesitate to send us you rewrite my essay for me or any other request.

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Hypothesis Testing in Healthcare Decision-Making Essay

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Introduction

Hypothesis testing plays a proficient role in the deductive and inductive derivation of insights across distinctive industries. Research indicates that developing a hypothesis in the healthcare sector is a statistical inference encompassing the comparative analysis of a causal or correlation perspective (Liu et al., 2022). Primarily, hypothesis testing is an initiative optimally utilized to quantify the strength of evidence parallel to the sample data. Therefore, the practice is a conductive factor in effective decision-making in medical care due to its attribution of establishing objective overviews on pertinent matters. The testing of a thesis is a strategic initiative that profoundly contributes to sustainable management and advancement in the healthcare mainframe.

Over the decades, the dynamism of sociocultural and economic practices fostered the emergence of various medical problems. One of the critical outliers to solving the challenges in the healthcare sector enshrines objectively demonstrating the interplay between the independent and dependent variables. Transcendentally, researchers agree that the use of hypothesis renders the identification of vital elements in the spectral issue while ascertaining the core approach to determine effective and functional decision-making among the practitioners (Prieto et al., 2021). An excellent example is a comparative analysis between obesity and lifestyle habits. The development of a hypothesis that addresses the pivotal effect between lifestyle habits and obesity further maps the trickle-down effect on individuals’ well-being.

In conclusion, it is the responsibility of professionals to exploit hypothesis testing strategies due to the objective effects. One of the primary virtues in the healthcare sector enshrines promoting the welfare of the patients. Therefore, it is critical to establish factorial baselines affirming correlations and causal perspectives. As a result, the stakeholders access ultimately actionable intelligence for sustainable decision-making. Hypothesis testing is an initiative that significantly influences the quality of medical care as a multidimensional phenomenon.

Liu, X., Zhang, Z., & Wang, L. (2022). Bayesian hypothesis testing of mediation: Methods and the impact of prior odds specifications . Behavior Research Methods , 1-13. Web.

Prieto, C., Kavetski, D., Le Vine, N., Álvarez, C., & Medina, R. (2021). Identification of dominant hydrological mechanisms using Bayesian inference, multiple statistical hypothesis testing, and flexible models . Water Resources Research , 57 (8), e2020WR028338. Web.

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Understanding Hypothesis Testing

Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

A hypothesis is an assumption or idea, specifically a statistical claim about an unknown population parameter. For example, a judge assumes a person is innocent and verifies this by reviewing evidence and hearing testimony before reaching a verdict.

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. 

To test the validity of the claim or assumption about the population parameter:

• A sample is drawn from the population and analyzed.
• The results of the analysis are used to decide whether the claim is true or not.

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

• Null hypothesis (H 0 ): In statistics, the null hypothesis is a general statement or default position that there is no relationship between two measured cases or no relationship among groups. In other words, it is a basic assumption or made based on the problem knowledge. Example : A company’s mean production is 50 units/per da H 0 : [Tex]\mu [/Tex] = 50.
• Alternative hypothesis (H 1 ): The alternative hypothesis is the hypothesis used in hypothesis testing that is contrary to the null hypothesis.  Example: A company’s production is not equal to 50 units/per day i.e. H 1 : [Tex]\mu [/Tex] [Tex]\ne [/Tex] 50.

Key Terms of Hypothesis Testing

• Level of significance : It refers to the degree of significance in which we accept or reject the null hypothesis. 100% accuracy is not possible for accepting a hypothesis, so we, therefore, select a level of significance that is usually 5%. This is normally denoted with  [Tex]\alpha[/Tex] and generally, it is 0.05 or 5%, which means your output should be 95% confident to give a similar kind of result in each sample.
• P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
• Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
• Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
• Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing. 

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

• Left-Tailed (Left-Sided) Test: The alternative hypothesis asserts that the true parameter value is less than the null hypothesis. Example: H 0 ​: [Tex]\mu \geq 50 [/Tex] and H 1 : [Tex]\mu < 50 [/Tex]
• Right-Tailed (Right-Sided) Test : The alternative hypothesis asserts that the true parameter value is greater than the null hypothesis. Example: H 0 : [Tex]\mu \leq50 [/Tex] and H 1 : [Tex]\mu > 50 [/Tex]

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

Example: H 0 : [Tex]\mu = [/Tex] 50 and H 1 : [Tex]\mu \neq 50 [/Tex]

To delve deeper into differences into both types of test: Refer to link

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

• Type I error: When we reject the null hypothesis, although that hypothesis was true. Type I error is denoted by alpha( [Tex]\alpha [/Tex] ).
• Type II errors : When we accept the null hypothesis, but it is false. Type II errors are denoted by beta( [Tex]\beta [/Tex] ).

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

State the null hypothesis ( [Tex]H_0 [/Tex] ), representing no effect, and the alternative hypothesis ( [Tex]H_1 [/Tex] ​), suggesting an effect or difference.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

Select a significance level ( [Tex]\alpha [/Tex] ), typically 0.05, to determine the threshold for rejecting the null hypothesis. It provides validity to our hypothesis test, ensuring that we have sufficient data to back up our claims. Usually, we determine our significance level beforehand of the test. The p-value is the criterion used to calculate our significance value.

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

• Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
• t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
• Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
• F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

• If Test Statistic>Critical Value: Reject the null hypothesis.
• If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

• If the p-value is less than or equal to the significance level i.e. ( [Tex]p\leq\alpha [/Tex] ), you reject the null hypothesis. This indicates that the observed results are unlikely to have occurred by chance alone, providing evidence in favor of the alternative hypothesis.
• If the p-value is greater than the significance level i.e. ( [Tex]p\geq \alpha[/Tex] ), you fail to reject the null hypothesis. This suggests that the observed results are consistent with what would be expected under the null hypothesis.

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

[Tex]z = \frac{\bar{x} – \mu}{\frac{\sigma}{\sqrt{n}}}[/Tex]

• [Tex]\bar{x} [/Tex] is the sample mean,
• μ represents the population mean, 
• σ is the standard deviation
• and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

[Tex]t=\frac{x̄-μ}{s/\sqrt{n}} [/Tex]

• t = t-score,
• x̄ = sample mean
• μ = population mean,
• s = standard deviation of the sample,
• n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

[Tex]\chi^2 = \sum \frac{(O_{ij} – E_{ij})^2}{E_{ij}}[/Tex]

• [Tex]O_{ij}[/Tex] is the observed frequency in cell [Tex]{ij} [/Tex]
• i,j are the rows and columns index respectively.
• [Tex]E_{ij}[/Tex] is the expected frequency in cell [Tex]{ij}[/Tex] , calculated as : [Tex]\frac{{\text{{Row total}} \times \text{{Column total}}}}{{\text{{Total observations}}}}[/Tex]

Real life Examples of Hypothesis Testing

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

• Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
• After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

• Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
• Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

• m  = mean of the difference i.e X after, X before
• s  = standard deviation of the difference (d) i.e d i ​= X after, i ​− X before,
• n  = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

• If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
• If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Case A

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

import numpy as np from scipy import stats # Data before_treatment = np . array ([ 120 , 122 , 118 , 130 , 125 , 128 , 115 , 121 , 123 , 119 ]) after_treatment = np . array ([ 115 , 120 , 112 , 128 , 122 , 125 , 110 , 117 , 119 , 114 ]) # Step 1: Null and Alternate Hypotheses # Null Hypothesis: The new drug has no effect on blood pressure. # Alternate Hypothesis: The new drug has an effect on blood pressure. null_hypothesis = "The new drug has no effect on blood pressure." alternate_hypothesis = "The new drug has an effect on blood pressure." # Step 2: Significance Level alpha = 0.05 # Step 3: Paired T-test t_statistic , p_value = stats . ttest_rel ( after_treatment , before_treatment ) # Step 4: Calculate T-statistic manually m = np . mean ( after_treatment - before_treatment ) s = np . std ( after_treatment - before_treatment , ddof = 1 ) # using ddof=1 for sample standard deviation n = len ( before_treatment ) t_statistic_manual = m / ( s / np . sqrt ( n )) # Step 5: Decision if p_value <= alpha : decision = "Reject" else : decision = "Fail to reject" # Conclusion if decision == "Reject" : conclusion = "There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different." else : conclusion = "There is insufficient evidence to claim a significant difference in average blood pressure before and after treatment with the new drug." # Display results print ( "T-statistic (from scipy):" , t_statistic ) print ( "P-value (from scipy):" , p_value ) print ( "T-statistic (calculated manually):" , t_statistic_manual ) print ( f "Decision: { decision } the null hypothesis at alpha= { alpha } ." ) print ( "Conclusion:" , conclusion )

T-statistic (from scipy): -9.0 P-value (from scipy): 8.538051223166285e-06 T-statistic (calculated manually): -9.0 Decision: Reject the null hypothesis at alpha=0.05. Conclusion: There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05. 

• The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
• The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

• Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
• Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

The test statistic is calculated by using the z formula Z = [Tex](203.8 – 200) / (5 \div \sqrt{25}) [/Tex] ​ and we get accordingly , Z =2.039999999999992.

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Python Implementation of Case B

import scipy.stats as stats import math import numpy as np # Given data sample_data = np . array ( [ 205 , 198 , 210 , 190 , 215 , 205 , 200 , 192 , 198 , 205 , 198 , 202 , 208 , 200 , 205 , 198 , 205 , 210 , 192 , 205 , 198 , 205 , 210 , 192 , 205 ]) population_std_dev = 5 population_mean = 200 sample_size = len ( sample_data ) # Step 1: Define the Hypotheses # Null Hypothesis (H0): The average cholesterol level in a population is 200 mg/dL. # Alternate Hypothesis (H1): The average cholesterol level in a population is different from 200 mg/dL. # Step 2: Define the Significance Level alpha = 0.05 # Two-tailed test # Critical values for a significance level of 0.05 (two-tailed) critical_value_left = stats . norm . ppf ( alpha / 2 ) critical_value_right = - critical_value_left # Step 3: Compute the test statistic sample_mean = sample_data . mean () z_score = ( sample_mean - population_mean ) / \ ( population_std_dev / math . sqrt ( sample_size )) # Step 4: Result # Check if the absolute value of the test statistic is greater than the critical values if abs ( z_score ) > max ( abs ( critical_value_left ), abs ( critical_value_right )): print ( "Reject the null hypothesis." ) print ( "There is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL." ) else : print ( "Fail to reject the null hypothesis." ) print ( "There is not enough evidence to conclude that the average cholesterol level in the population is different from 200 mg/dL." )

Reject the null hypothesis. There is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL.

Limitations of Hypothesis Testing

• Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
• The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
• Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( [Tex]H_o [/Tex] ): No effect or difference exists. Alternative Hypothesis ( [Tex]H_1 [/Tex] ): An effect or difference exists. Significance Level ( [Tex]\alpha [/Tex] ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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