example significance of the study in research paper

How To Write Significance of the Study (With Examples) 

Whether you’re writing a research paper or thesis, a portion called Significance of the Study ensures your readers understand the impact of your work. Learn how to effectively write this vital part of your research paper or thesis through our detailed steps, guidelines, and examples.

Related: How to Write a Concept Paper for Academic Research

Table of Contents

What is the significance of the study.

The Significance of the Study presents the importance of your research. It allows you to prove the study’s impact on your field of research, the new knowledge it contributes, and the people who will benefit from it.

Related: How To Write Scope and Delimitation of a Research Paper (With Examples)

Where Should I Put the Significance of the Study?

The Significance of the Study is part of the first chapter or the Introduction. It comes after the research’s rationale, problem statement, and hypothesis.

Related: How to Make Conceptual Framework (with Examples and Templates)

Why Should I Include the Significance of the Study?

The purpose of the Significance of the Study is to give you space to explain to your readers how exactly your research will be contributing to the literature of the field you are studying 1 . It’s where you explain why your research is worth conducting and its significance to the community, the people, and various institutions.

How To Write Significance of the Study: 5 Steps

Below are the steps and guidelines for writing your research’s Significance of the Study.

1. Use Your Research Problem as a Starting Point

Your problem statement can provide clues to your research study’s outcome and who will benefit from it 2 .

Ask yourself, “How will the answers to my research problem be beneficial?”. In this manner, you will know how valuable it is to conduct your study. 

Let’s say your research problem is “What is the level of effectiveness of the lemongrass (Cymbopogon citratus) in lowering the blood glucose level of Swiss mice (Mus musculus)?”

Discovering a positive correlation between the use of lemongrass and lower blood glucose level may lead to the following results:

• Increased public understanding of the plant’s medical properties;
• Higher appreciation of the importance of lemongrass  by the community;
• Adoption of lemongrass tea as a cheap, readily available, and natural remedy to lower their blood glucose level.

Once you’ve zeroed in on the general benefits of your study, it’s time to break it down into specific beneficiaries.

2. State How Your Research Will Contribute to the Existing Literature in the Field

Think of the things that were not explored by previous studies. Then, write how your research tackles those unexplored areas. Through this, you can convince your readers that you are studying something new and adding value to the field.

3. Explain How Your Research Will Benefit Society

In this part, tell how your research will impact society. Think of how the results of your study will change something in your community. 

For example, in the study about using lemongrass tea to lower blood glucose levels, you may indicate that through your research, the community will realize the significance of lemongrass and other herbal plants. As a result, the community will be encouraged to promote the cultivation and use of medicinal plants.

4. Mention the Specific Persons or Institutions Who Will Benefit From Your Study

Using the same example above, you may indicate that this research’s results will benefit those seeking an alternative supplement to prevent high blood glucose levels.

5. Indicate How Your Study May Help Future Studies in the Field

You must also specifically indicate how your research will be part of the literature of your field and how it will benefit future researchers. In our example above, you may indicate that through the data and analysis your research will provide, future researchers may explore other capabilities of herbal plants in preventing different diseases.

Tips and Warnings

• Think ahead . By visualizing your study in its complete form, it will be easier for you to connect the dots and identify the beneficiaries of your research.
• Write concisely. Make it straightforward, clear, and easy to understand so that the readers will appreciate the benefits of your research. Avoid making it too long and wordy.
• Go from general to specific . Like an inverted pyramid, you start from above by discussing the general contribution of your study and become more specific as you go along. For instance, if your research is about the effect of remote learning setup on the mental health of college students of a specific university , you may start by discussing the benefits of the research to society, to the educational institution, to the learning facilitators, and finally, to the students.
• Seek help . For example, you may ask your research adviser for insights on how your research may contribute to the existing literature. If you ask the right questions, your research adviser can point you in the right direction.
• Revise, revise, revise. Be ready to apply necessary changes to your research on the fly. Unexpected things require adaptability, whether it’s the respondents or variables involved in your study. There’s always room for improvement, so never assume your work is done until you have reached the finish line.

Significance of the Study Examples

This section presents examples of the Significance of the Study using the steps and guidelines presented above.

Example 1: STEM-Related Research

Research Topic: Level of Effectiveness of the Lemongrass ( Cymbopogon citratus ) Tea in Lowering the Blood Glucose Level of Swiss Mice ( Mus musculus ).

Significance of the Study .

This research will provide new insights into the medicinal benefit of lemongrass ( Cymbopogon citratus ), specifically on its hypoglycemic ability.

Through this research, the community will further realize promoting medicinal plants, especially lemongrass, as a preventive measure against various diseases. People and medical institutions may also consider lemongrass tea as an alternative supplement against hyperglycemia. 

Moreover, the analysis presented in this study will convey valuable information for future research exploring the medicinal benefits of lemongrass and other medicinal plants.  

Example 2: Business and Management-Related Research

Research Topic: A Comparative Analysis of Traditional and Social Media Marketing of Small Clothing Enterprises.

Significance of the Study:

By comparing the two marketing strategies presented by this research, there will be an expansion on the current understanding of the firms on these marketing strategies in terms of cost, acceptability, and sustainability. This study presents these marketing strategies for small clothing enterprises, giving them insights into which method is more appropriate and valuable for them. 

Specifically, this research will benefit start-up clothing enterprises in deciding which marketing strategy they should employ. Long-time clothing enterprises may also consider the result of this research to review their current marketing strategy.

Furthermore, a detailed presentation on the comparison of the marketing strategies involved in this research may serve as a tool for further studies to innovate the current method employed in the clothing Industry.

Example 3: Social Science -Related Research.

Research Topic:  Divide Et Impera : An Overview of How the Divide-and-Conquer Strategy Prevailed on Philippine Political History.

Significance of the Study :

Through the comprehensive exploration of this study on Philippine political history, the influence of the Divide et Impera, or political decentralization, on the political discernment across the history of the Philippines will be unraveled, emphasized, and scrutinized. Moreover, this research will elucidate how this principle prevailed until the current political theatre of the Philippines.

In this regard, this study will give awareness to society on how this principle might affect the current political context. Moreover, through the analysis made by this study, political entities and institutions will have a new approach to how to deal with this principle by learning about its influence in the past.

In addition, the overview presented in this research will push for new paradigms, which will be helpful for future discussion of the Divide et Impera principle and may lead to a more in-depth analysis.

Example 4: Humanities-Related Research

Research Topic: Effectiveness of Meditation on Reducing the Anxiety Levels of College Students.

Significance of the Study: 

This research will provide new perspectives in approaching anxiety issues of college students through meditation. 

Specifically, this research will benefit the following:

 Community – this study spreads awareness on recognizing anxiety as a mental health concern and how meditation can be a valuable approach to alleviating it.

Academic Institutions and Administrators – through this research, educational institutions and administrators may promote programs and advocacies regarding meditation to help students deal with their anxiety issues.

Mental health advocates – the result of this research will provide valuable information for the advocates to further their campaign on spreading awareness on dealing with various mental health issues, including anxiety, and how to stop stigmatizing those with mental health disorders.

Parents – this research may convince parents to consider programs involving meditation that may help the students deal with their anxiety issues.

Students will benefit directly from this research as its findings may encourage them to consider meditation to lower anxiety levels.

Future researchers – this study covers information involving meditation as an approach to reducing anxiety levels. Thus, the result of this study can be used for future discussions on the capabilities of meditation in alleviating other mental health concerns.

Frequently Asked Questions

1. what is the difference between the significance of the study and the rationale of the study.

Both aim to justify the conduct of the research. However, the Significance of the Study focuses on the specific benefits of your research in the field, society, and various people and institutions. On the other hand, the Rationale of the Study gives context on why the researcher initiated the conduct of the study.

Let’s take the research about the Effectiveness of Meditation in Reducing Anxiety Levels of College Students as an example. Suppose you are writing about the Significance of the Study. In that case, you must explain how your research will help society, the academic institution, and students deal with anxiety issues through meditation. Meanwhile, for the Rationale of the Study, you may state that due to the prevalence of anxiety attacks among college students, you’ve decided to make it the focal point of your research work.

2. What is the difference between Justification and the Significance of the Study?

In Justification, you express the logical reasoning behind the conduct of the study. On the other hand, the Significance of the Study aims to present to your readers the specific benefits your research will contribute to the field you are studying, community, people, and institutions.

Suppose again that your research is about the Effectiveness of Meditation in Reducing the Anxiety Levels of College Students. Suppose you are writing the Significance of the Study. In that case, you may state that your research will provide new insights and evidence regarding meditation’s ability to reduce college students’ anxiety levels. Meanwhile, you may note in the Justification that studies are saying how people used meditation in dealing with their mental health concerns. You may also indicate how meditation is a feasible approach to managing anxiety using the analysis presented by previous literature.

3. How should I start my research’s Significance of the Study section?

– This research will contribute… – The findings of this research… – This study aims to… – This study will provide… – Through the analysis presented in this study… – This study will benefit…

Moreover, you may start the Significance of the Study by elaborating on the contribution of your research in the field you are studying.

4. What is the difference between the Purpose of the Study and the Significance of the Study?

The Purpose of the Study focuses on why your research was conducted, while the Significance of the Study tells how the results of your research will benefit anyone.

Suppose your research is about the Effectiveness of Lemongrass Tea in Lowering the Blood Glucose Level of Swiss Mice . You may include in your Significance of the Study that the research results will provide new information and analysis on the medical ability of lemongrass to solve hyperglycemia. Meanwhile, you may include in your Purpose of the Study that your research wants to provide a cheaper and natural way to lower blood glucose levels since commercial supplements are expensive.

5. What is the Significance of the Study in Tagalog?

In Filipino research, the Significance of the Study is referred to as Kahalagahan ng Pag-aaral.

• Draft your Significance of the Study. Retrieved 18 April 2021, from http://dissertationedd.usc.edu/draft-your-significance-of-the-study.html
• Regoniel, P. (2015). Two Tips on How to Write the Significance of the Study. Retrieved 18 April 2021, from https://simplyeducate.me/2015/02/09/significance-of-the-study/

Written by Jewel Kyle Fabula

in Career and Education , Juander How

Jewel Kyle Fabula

Jewel Kyle Fabula is a Bachelor of Science in Economics student at the University of the Philippines Diliman. His passion for learning mathematics developed as he competed in some mathematics competitions during his Junior High School years. He loves cats, playing video games, and listening to music.

Browse all articles written by Jewel Kyle Fabula

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The Savvy Scientist

Experiences of a London PhD student and beyond

What is the Significance of a Study? Examples and Guide

If you’re reading this post you’re probably wondering: what is the significance of a study?

No matter where you’re at with a piece of research, it is a good idea to think about the potential significance of your work. And sometimes you’ll have to explicitly write a statement of significance in your papers, it addition to it forming part of your thesis.

In this post I’ll cover what the significance of a study is, how to measure it, how to describe it with examples and add in some of my own experiences having now worked in research for over nine years.

If you’re reading this because you’re writing up your first paper, welcome! You may also like my how-to guide for all aspects of writing your first research paper .

Looking for guidance on writing the statement of significance for a paper or thesis? Click here to skip straight to that section.

What is the Significance of a Study?

For research papers, theses or dissertations it’s common to explicitly write a section describing the significance of the study. We’ll come onto what to include in that section in just a moment.

However the significance of a study can actually refer to several different things.

Working our way from the most technical to the broadest, depending on the context, the significance of a study may refer to:

• Within your study: Statistical significance. Can we trust the findings?
• Wider research field: Research significance. How does your study progress the field?
• Commercial / economic significance: Could there be business opportunities for your findings?
• Societal significance: What impact could your study have on the wider society.
• And probably other domain-specific significance!

We’ll shortly cover each of them in turn, including how they’re measured and some examples for each type of study significance.

But first, let’s touch on why you should consider the significance of your research at an early stage.

Why Care About the Significance of a Study?

No matter what is motivating you to carry out your research, it is sensible to think about the potential significance of your work. In the broadest sense this asks, how does the study contribute to the world?

After all, for many people research is only worth doing if it will result in some expected significance. For the vast majority of us our studies won’t be significant enough to reach the evening news, but most studies will help to enhance knowledge in a particular field and when research has at least some significance it makes for a far more fulfilling longterm pursuit.

Furthermore, a lot of us are carrying out research funded by the public. It therefore makes sense to keep an eye on what benefits the work could bring to the wider community.

Often in research you’ll come to a crossroads where you must decide which path of research to pursue. Thinking about the potential benefits of a strand of research can be useful for deciding how to spend your time, money and resources.

It’s worth noting though, that not all research activities have to work towards obvious significance. This is especially true while you’re a PhD student, where you’re figuring out what you enjoy and may simply be looking for an opportunity to learn a new skill.

However, if you’re trying to decide between two potential projects, it can be useful to weigh up the potential significance of each.

Let’s now dive into the different types of significance, starting with research significance.

Research Significance

What is the research significance of a study.

Unless someone specifies which type of significance they’re referring to, it is fair to assume that they want to know about the research significance of your study.

Research significance describes how your work has contributed to the field, how it could inform future studies and progress research.

Where should I write about my study’s significance in my thesis?

Typically you should write about your study’s significance in the Introduction and Conclusions sections of your thesis.

It’s important to mention it in the Introduction so that the relevance of your work and the potential impact and benefits it could have on the field are immediately apparent. Explaining why your work matters will help to engage readers (and examiners!) early on.

It’s also a good idea to detail the study’s significance in your Conclusions section. This adds weight to your findings and helps explain what your study contributes to the field.

On occasion you may also choose to include a brief description in your Abstract.

What is expected when submitting an article to a journal

It is common for journals to request a statement of significance, although this can sometimes be called other things such as:

• Impact statement
• Significance statement
• Advances in knowledge section

Here is one such example of what is expected:

Impact Statement:  An Impact Statement is required for all submissions.  Your impact statement will be evaluated by the Editor-in-Chief, Global Editors, and appropriate Associate Editor. For your manuscript to receive full review, the editors must be convinced that it is an important advance in for the field. The Impact Statement is not a restating of the abstract. It should address the following: Why is the work submitted important to the field? How does the work submitted advance the field? What new information does this work impart to the field? How does this new information impact the field? Experimental Biology and Medicine journal, author guidelines

Typically the impact statement will be shorter than the Abstract, around 150 words.

Defining the study’s significance is helpful not just for the impact statement (if the journal asks for one) but also for building a more compelling argument throughout your submission. For instance, usually you’ll start the Discussion section of a paper by highlighting the research significance of your work. You’ll also include a short description in your Abstract too.

How to describe the research significance of a study, with examples

Whether you’re writing a thesis or a journal article, the approach to writing about the significance of a study are broadly the same.

I’d therefore suggest using the questions above as a starting point to base your statements on.

• Why is the work submitted important to the field?
• How does the work submitted advance the field?
• What new information does this work impart to the field?
• How does this new information impact the field?

Answer those questions and you’ll have a much clearer idea of the research significance of your work.

When describing it, try to clearly state what is novel about your study’s contribution to the literature. Then go on to discuss what impact it could have on progressing the field along with recommendations for future work.

Potential sentence starters

If you’re not sure where to start, why not set a 10 minute timer and have a go at trying to finish a few of the following sentences. Not sure on what to put? Have a chat to your supervisor or lab mates and they may be able to suggest some ideas.

• This study is important to the field because…
• These findings advance the field by…
• Our results highlight the importance of…
• Our discoveries impact the field by…

Now you’ve had a go let’s have a look at some real life examples.

Statement of significance examples

A statement of significance / impact:

Impact Statement This review highlights the historical development of the concept of “ideal protein” that began in the 1950s and 1980s for poultry and swine diets, respectively, and the major conceptual deficiencies of the long-standing concept of “ideal protein” in animal nutrition based on recent advances in amino acid (AA) metabolism and functions. Nutritionists should move beyond the “ideal protein” concept to consider optimum ratios and amounts of all proteinogenic AAs in animal foods and, in the case of carnivores, also taurine. This will help formulate effective low-protein diets for livestock, poultry, and fish, while sustaining global animal production. Because they are not only species of agricultural importance, but also useful models to study the biology and diseases of humans as well as companion (e.g. dogs and cats), zoo, and extinct animals in the world, our work applies to a more general readership than the nutritionists and producers of farm animals. Wu G, Li P. The “ideal protein” concept is not ideal in animal nutrition.  Experimental Biology and Medicine . 2022;247(13):1191-1201. doi: 10.1177/15353702221082658

And the same type of section but this time called “Advances in knowledge”:

Advances in knowledge: According to the MY-RADs criteria, size measurements of focal lesions in MRI are now of relevance for response assessment in patients with monoclonal plasma cell disorders. Size changes of 1 or 2 mm are frequently observed due to uncertainty of the measurement only, while the actual focal lesion has not undergone any biological change. Size changes of at least 6 mm or more in  T 1  weighted or  T 2  weighted short tau inversion recovery sequences occur in only 5% or less of cases when the focal lesion has not undergone any biological change. Wennmann M, Grözinger M, Weru V, et al. Test-retest, inter- and intra-rater reproducibility of size measurements of focal bone marrow lesions in MRI in patients with multiple myeloma [published online ahead of print, 2023 Apr 12].  Br J Radiol . 2023;20220745. doi: 10.1259/bjr.20220745

Other examples of research significance

Moving beyond the formal statement of significance, here is how you can describe research significance more broadly within your paper.

Describing research impact in an Abstract of a paper:

Three-dimensional visualisation and quantification of the chondrocyte population within articular cartilage can be achieved across a field of view of several millimetres using laboratory-based micro-CT. The ability to map chondrocytes in 3D opens possibilities for research in fields from skeletal development through to medical device design and treatment of cartilage degeneration. Conclusions section of the abstract in my first paper .

In the Discussion section of a paper:

We report for the utility of a standard laboratory micro-CT scanner to visualise and quantify features of the chondrocyte population within intact articular cartilage in 3D. This study represents a complimentary addition to the growing body of evidence supporting the non-destructive imaging of the constituents of articular cartilage. This offers researchers the opportunity to image chondrocyte distributions in 3D without specialised synchrotron equipment, enabling investigations such as chondrocyte morphology across grades of cartilage damage, 3D strain mapping techniques such as digital volume correlation to evaluate mechanical properties  in situ , and models for 3D finite element analysis  in silico  simulations. This enables an objective quantification of chondrocyte distribution and morphology in three dimensions allowing greater insight for investigations into studies of cartilage development, degeneration and repair. One such application of our method, is as a means to provide a 3D pattern in the cartilage which, when combined with digital volume correlation, could determine 3D strain gradient measurements enabling potential treatment and repair of cartilage degeneration. Moreover, the method proposed here will allow evaluation of cartilage implanted with tissue engineered scaffolds designed to promote chondral repair, providing valuable insight into the induced regenerative process. The Discussion section of the paper is laced with references to research significance.

How is longer term research significance measured?

Looking beyond writing impact statements within papers, sometimes you’ll want to quantify the long term research significance of your work. For instance when applying for jobs.

The most obvious measure of a study’s long term research significance is the number of citations it receives from future publications. The thinking is that a study which receives more citations will have had more research impact, and therefore significance , than a study which received less citations. Citations can give a broad indication of how useful the work is to other researchers but citations aren’t really a good measure of significance.

Bear in mind that us researchers can be lazy folks and sometimes are simply looking to cite the first paper which backs up one of our claims. You can find studies which receive a lot of citations simply for packaging up the obvious in a form which can be easily found and referenced, for instance by having a catchy or optimised title.

Likewise, research activity varies wildly between fields. Therefore a certain study may have had a big impact on a particular field but receive a modest number of citations, simply because not many other researchers are working in the field.

Nevertheless, citations are a standard measure of significance and for better or worse it remains impressive for someone to be the first author of a publication receiving lots of citations.

Other measures for the research significance of a study include:

• Accolades: best paper awards at conferences, thesis awards, “most downloaded” titles for articles, press coverage.
• How much follow-on research the study creates. For instance, part of my PhD involved a novel material initially developed by another PhD student in the lab. That PhD student’s research had unlocked lots of potential new studies and now lots of people in the group were using the same material and developing it for different applications. The initial study may not receive a high number of citations yet long term it generated a lot of research activity.

That covers research significance, but you’ll often want to consider other types of significance for your study and we’ll cover those next.

Statistical Significance

What is the statistical significance of a study.

Often as part of a study you’ll carry out statistical tests and then state the statistical significance of your findings: think p-values eg <0.05. It is useful to describe the outcome of these tests within your report or paper, to give a measure of statistical significance.

Effectively you are trying to show whether the performance of your innovation is actually better than a control or baseline and not just chance. Statistical significance deserves a whole other post so I won’t go into a huge amount of depth here.

Things that make publication in  The BMJ  impossible or unlikely Internal validity/robustness of the study • It had insufficient statistical power, making interpretation difficult; • Lack of statistical power; The British Medical Journal’s guide for authors

Calculating statistical significance isn’t always necessary (or valid) for a study, such as if you have a very small number of samples, but it is a very common requirement for scientific articles.

Writing a journal article? Check the journal’s guide for authors to see what they expect. Generally if you have approximately five or more samples or replicates it makes sense to start thinking about statistical tests. Speak to your supervisor and lab mates for advice, and look at other published articles in your field.

How is statistical significance measured?

Statistical significance is quantified using p-values . Depending on your study design you’ll choose different statistical tests to compute the p-value.

A p-value of 0.05 is a common threshold value. The 0.05 means that there is a 1/20 chance that the difference in performance you’re reporting is just down to random chance.

• p-values above 0.05 mean that the result isn’t statistically significant enough to be trusted: it is too likely that the effect you’re showing is just luck.
• p-values less than or equal to 0.05 mean that the result is statistically significant. In other words: unlikely to just be chance, which is usually considered a good outcome.

Low p-values (eg p = 0.001) mean that it is highly unlikely to be random chance (1/1000 in the case of p = 0.001), therefore more statistically significant.

It is important to clarify that, although low p-values mean that your findings are statistically significant, it doesn’t automatically mean that the result is scientifically important. More on that in the next section on research significance.

How to describe the statistical significance of your study, with examples

In the first paper from my PhD I ran some statistical tests to see if different staining techniques (basically dyes) increased how well you could see cells in cow tissue using micro-CT scanning (a 3D imaging technique).

In your methods section you should mention the statistical tests you conducted and then in the results you will have statements such as:

Between mediums for the two scan protocols C/N [contrast to noise ratio] was greater for EtOH than the PBS in both scanning methods (both  p  < 0.0001) with mean differences of 1.243 (95% CI [confidence interval] 0.709 to 1.778) for absorption contrast and 6.231 (95% CI 5.772 to 6.690) for propagation contrast. … Two repeat propagation scans were taken of samples from the PTA-stained groups. No difference in mean C/N was found with either medium: PBS had a mean difference of 0.058 ( p  = 0.852, 95% CI -0.560 to 0.676), EtOH had a mean difference of 1.183 ( p  = 0.112, 95% CI 0.281 to 2.648). From the Results section of my first paper, available here . Square brackets added for this post to aid clarity.

From this text the reader can infer from the first paragraph that there was a statistically significant difference in using EtOH compared to PBS (really small p-value of <0.0001). However, from the second paragraph, the difference between two repeat scans was statistically insignificant for both PBS (p = 0.852) and EtOH (p = 0.112).

By conducting these statistical tests you have then earned your right to make bold statements, such as these from the discussion section:

Propagation phase-contrast increases the contrast of individual chondrocytes [cartilage cells] compared to using absorption contrast. From the Discussion section from the same paper.

Without statistical tests you have no evidence that your results are not just down to random chance.

Beyond describing the statistical significance of a study in the main body text of your work, you can also show it in your figures.

In figures such as bar charts you’ll often see asterisks to represent statistical significance, and “n.s.” to show differences between groups which are not statistically significant. Here is one such figure, with some subplots, from the same paper:

In this example an asterisk (*) between two bars represents p < 0.05. Two asterisks (**) represents p < 0.001 and three asterisks (***) represents p < 0.0001. This should always be stated in the caption of your figure since the values that each asterisk refers to can vary.

Now that we know if a study is showing statistically and research significance, let’s zoom out a little and consider the potential for commercial significance.

Commercial and Industrial Significance

What are commercial and industrial significance.

Moving beyond significance in relation to academia, your research may also have commercial or economic significance.

Simply put:

• Commercial significance: could the research be commercialised as a product or service? Perhaps the underlying technology described in your study could be licensed to a company or you could even start your own business using it.
• Industrial significance: more widely than just providing a product which could be sold, does your research provide insights which may affect a whole industry? Such as: revealing insights or issues with current practices, performance gains you don’t want to commercialise (e.g. solar power efficiency), providing suggested frameworks or improvements which could be employed industry-wide.

I’ve grouped these two together because there can certainly be overlap. For instance, perhaps your new technology could be commercialised whilst providing wider improvements for the whole industry.

Commercial and industrial significance are not relevant to most studies, so only write about it if you and your supervisor can think of reasonable routes to your work having an impact in these ways.

How are commercial and industrial significance measured?

Unlike statistical and research significances, the measures of commercial and industrial significance can be much more broad.

Here are some potential measures of significance:

Commercial significance:

• How much value does your technology bring to potential customers or users?
• How big is the potential market and how much revenue could the product potentially generate?
• Is the intellectual property protectable? i.e. patentable, or if not could the novelty be protected with trade secrets: if so publish your method with caution!
• If commercialised, could the product bring employment to a geographical area?

Industrial significance:

What impact could it have on the industry? For instance if you’re revealing an issue with something, such as unintended negative consequences of a drug , what does that mean for the industry and the public? This could be:

• Reduced overhead costs
• Better safety
• Faster production methods
• Improved scaleability

How to describe the commercial and industrial significance of a study, with examples

Commercial significance.

If your technology could be commercially viable, and you’ve got an interest in commercialising it yourself, it is likely that you and your university may not want to immediately publish the study in a journal.

You’ll probably want to consider routes to exploiting the technology and your university may have a “technology transfer” team to help researchers navigate the various options.

However, if instead of publishing a paper you’re submitting a thesis or dissertation then it can be useful to highlight the commercial significance of your work. In this instance you could include statements of commercial significance such as:

The measurement technology described in this study provides state of the art performance and could enable the development of low cost devices for aerospace applications. An example of commercial significance I invented for this post

Industrial significance

First, think about the industrial sectors who could benefit from the developments described in your study.

For example if you’re working to improve battery efficiency it is easy to think of how it could lead to performance gains for certain industries, like personal electronics or electric vehicles. In these instances you can describe the industrial significance relatively easily, based off your findings.

For example:

By utilising abundant materials in the described battery fabrication process we provide a framework for battery manufacturers to reduce dependence on rare earth components. Again, an invented example

For other technologies there may well be industrial applications but they are less immediately obvious and applicable. In these scenarios the best you can do is to simply reframe your research significance statement in terms of potential commercial applications in a broad way.

As a reminder: not all studies should address industrial significance, so don’t try to invent applications just for the sake of it!

Societal Significance

What is the societal significance of a study.

The most broad category of significance is the societal impact which could stem from it.

If you’re working in an applied field it may be quite easy to see a route for your research to impact society. For others, the route to societal significance may be less immediate or clear.

Studies can help with big issues facing society such as:

• Medical applications : vaccines, surgical implants, drugs, improving patient safety. For instance this medical device and drug combination I worked on which has a very direct route to societal significance.
• Political significance : Your research may provide insights which could contribute towards potential changes in policy or better understanding of issues facing society.
• Public health : for instance COVID-19 transmission and related decisions.
• Climate change : mitigation such as more efficient solar panels and lower cost battery solutions, and studying required adaptation efforts and technologies. Also, better understanding around related societal issues, for instance this study on the effects of temperature on hate speech.

How is societal significance measured?

Societal significance at a high level can be quantified by the size of its potential societal effect. Just like a lab risk assessment, you can think of it in terms of probability (or how many people it could help) and impact magnitude.

Societal impact = How many people it could help x the magnitude of the impact

Think about how widely applicable the findings are: for instance does it affect only certain people? Then think about the potential size of the impact: what kind of difference could it make to those people?

Between these two metrics you can get a pretty good overview of the potential societal significance of your research study.

How to describe the societal significance of a study, with examples

Quite often the broad societal significance of your study is what you’re setting the scene for in your Introduction. In addition to describing the existing literature, it is common to for the study’s motivation to touch on its wider impact for society.

For those of us working in healthcare research it is usually pretty easy to see a path towards societal significance.

Our CLOUT model has state-of-the-art performance in mortality prediction, surpassing other competitive NN models and a logistic regression model … Our results show that the risk factors identified by the CLOUT model agree with physicians’ assessment, suggesting that CLOUT could be used in real-world clinicalsettings. Our results strongly support that CLOUT may be a useful tool to generate clinical prediction models, especially among hospitalized and critically ill patient populations. Learning Latent Space Representations to Predict Patient Outcomes: Model Development and Validation

In other domains the societal significance may either take longer or be more indirect, meaning that it can be more difficult to describe the societal impact.

Even so, here are some examples I’ve found from studies in non-healthcare domains:

We examined food waste as an initial investigation and test of this methodology, and there is clear potential for the examination of not only other policy texts related to food waste (e.g., liability protection, tax incentives, etc.; Broad Leib et al., 2020) but related to sustainable fishing (Worm et al., 2006) and energy use (Hawken, 2017). These other areas are of obvious relevance to climate change… AI-Based Text Analysis for Evaluating Food Waste Policies

The continued development of state-of-the art NLP tools tailored to climate policy will allow climate researchers and policy makers to extract meaningful information from this growing body of text, to monitor trends over time and administrative units, and to identify potential policy improvements. BERT Classification of Paris Agreement Climate Action Plans

Top Tips For Identifying & Writing About the Significance of Your Study

• Writing a thesis? Describe the significance of your study in the Introduction and the Conclusion .
• Submitting a paper? Read the journal’s guidelines. If you’re writing a statement of significance for a journal, make sure you read any guidance they give for what they’re expecting.
• Take a step back from your research and consider your study’s main contributions.
• Read previously published studies in your field . Use this for inspiration and ideas on how to describe the significance of your own study
• Discuss the study with your supervisor and potential co-authors or collaborators and brainstorm potential types of significance for it.

Now you’ve finished reading up on the significance of a study you may also like my how-to guide for all aspects of writing your first research paper .

Writing an academic journal paper

I hope that you’ve learned something useful from this article about the significance of a study. If you have any more research-related questions let me know, I’m here to help.

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Significance of the Study

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The significance of the study underscores the research’s importance, illustrating its impact on existing knowledge and potential applications. It highlights how the findings address gaps, resolve problems, or contribute to advancements in a specific field. By emphasizing the study’s relevance, it demonstrates the broader implications for society, academia, or industry, justifying the research effort and investment.

What is the Significance of the Study?

The significance of the study illustrates the research’s importance, highlighting its impact on existing knowledge and potential applications. It addresses gaps, resolves problems, or contributes to advancements in a specific field. Emphasizing the study’s relevance, it demonstrates broader implications for society, academia, or industry, justifying the research effort and investment.

Significance of the Study Format

When writing the “Significance of the Study” section in a research paper , follow this format to ensure clarity and impact:

1. Introduction

• Contextual Background: Provide a brief background of the research topic.
• Research Problem: State the problem the study addresses.

2. Purpose of the Study

• Objective Statement: Clearly define the main objective of the study.
• Scope of the Study: Outline what the study covers.

3. Importance to the Field

• Contribution to Knowledge: Explain how the study will add to existing knowledge.
• Theoretical Significance: Discuss the study’s theoretical implications.

4. Practical Implications

• Real-world Application: Describe how the findings can be applied in practical setting .
• Beneficiaries: Identify who will benefit from the research (e.g., policymakers, practitioners, educators).

5. Advancement of Future Research

• Foundation for Future Studies: Indicate how the study can serve as a basis for further research.
• Research Gaps: Highlight any gaps the study aims to fill.

6. Societal Impact

• Broader Implications: Discuss the potential societal benefits or changes resulting from the study.
• Public Awareness: Explain how the study can raise awareness or understanding of the issue.

7. Conclusion

• Summary of Significance: Recap the main points that underline the importance of the study.
• Call to Action: Encourage specific actions or further studies based on the research findings.

Significance of the Study on Impact of Remote Work on Employee Productivity in the Tech Industry 1. Introduction The rapid shift to remote work due to the COVID-19 pandemic has fundamentally changed the dynamics of workplace productivity, especially within the tech industry. This study aims to examine how remote work influences employee productivity compared to traditional office settings. 2. Purpose of the Study The primary objective of this research is to evaluate the productivity levels of tech employees working remotely versus those working in office environments. The study analyzes various productivity metrics, such as task completion rates, quality of work, and employee satisfaction. 3. Importance to the Field This research contributes significantly to the existing body of knowledge by providing empirical data on the productivity impacts of remote work. It refines theoretical models of workplace productivity and offers new insights into remote work dynamics specific to the tech sector. Understanding these dynamics helps scholars and practitioners alike in shaping effective productivity strategies in the evolving work landscape. 4. Practical Implications The findings from this study have crucial practical implications for tech companies aiming to optimize their remote work policies. By understanding how remote work affects productivity, managers and HR departments can develop strategies to enhance employee performance and well-being in remote settings. These insights can also assist in designing training programs that equip employees with the skills needed for effective remote work. 5. Advancement of Future Research This study sets the stage for future research on long-term remote work trends and their impacts across various industries. It addresses existing gaps by providing a detailed analysis of how remote work influences productivity in the tech sector. Future researchers can build on this work to explore remote work dynamics in other fields and under different conditions. 6. Societal Impact The study highlights the broader societal implications of remote work, such as promoting work-life balance, reducing urban congestion, and lowering environmental pollution. By demonstrating the potential benefits of remote work, this research can influence public policy and corporate strategies towards more sustainable and flexible working conditions, ultimately contributing to societal well-being. 7. Conclusion Understanding the impact of remote work on productivity is essential for developing effective work policies and creating healthier work environments. This study provides valuable insights that can guide tech companies in optimizing their remote work strategies. Future research should explore the long-term effects of remote work across different sectors to provide a comprehensive understanding of its benefits and challenges.

Significance of the Study Examples

• Significance of the Study: Research Paper
• Significance of the Study: Qunatitive Research
• Significance of the Study: Qualitative Research

Research Paper

Qunatitive Research

Qualitative Research

More Significance of the Study Examples

• Educational Resources and Student Performance
• Business Innovation and Competitive Advantage
• Social Media Influencers and Brand Loyalty
• Mental Health Benefits of Physical Activ ity
• Sustainable Food Practices and Consumer Behavior
• Green Building and Energy Efficiency
• Technology in Healthcare
• Employee Engagement and Job Performance
• Business Strategies and Market Adaptation
• Mindfulness at Work

Purpose of Writing the Significance of a Study

When writing academic research or scholarly articles, one critical section is the significance of the study . This part addresses the importance and impact of the research, both theoretically and practically. Here are the main purposes of writing the significance of a study:

1. Establishing Relevance

The primary purpose is to explain why the study is relevant. It connects the research to existing literature, highlighting gaps or deficiencies that the current study aims to fill. This helps to justify the research problem and demonstrates the necessity of the study.

2. Highlighting Contributions

This section outlines the contributions the study will make to the field. It discusses how the findings can advance knowledge, theory, or practice. The significance emphasizes new insights, innovative approaches, or advancements that the study will provide.

3. Guiding Further Research

The significance of the study often includes suggestions for future research. By identifying limitations and unexplored areas, it encourages other researchers to pursue related questions. This helps to build a foundation for continuous inquiry and discovery.

4. Demonstrating Practical Applications

Beyond theoretical contributions, the significance of the study highlights practical applications. It shows how the research can solve real-world problems, improve practices, or influence policy-making. This connects academic research to practical outcomes that benefit society.

5. Engaging Stakeholders

Writing the significance of a study engages various stakeholders, including scholars, practitioners, policymakers, and funders. It communicates the value of the research to different audiences, making it easier to garner support, funding, or collaboration.

6. Enhancing Research Impact

A well-articulated significance section enhances the overall impact of the research. It underscores the importance and potential influence of the study, increasing its visibility and recognition in the academic community and beyond.

Benefits of Significance of the Study

Writing the significance of a study offers several benefits that enhance the research’s value and impact. Here are the key benefits:

1. Clarifies Research Value

The significance section clarifies the value of the research by explaining its importance and relevance. It helps readers understand why the study matters and what contributions it aims to make to the field.

2. Justifies the Research Problem

This section provides a rationale for the study by highlighting the research problem’s importance. It justifies the need for the study by identifying gaps in existing literature and explaining how the research will address these gaps.

3. Engages and Motivates Readers

A well-articulated significance section engages and motivates readers, including scholars, practitioners, and policymakers. It draws their interest by showcasing the study’s potential impact and benefits.

4. Secures Funding and Support

Explaining the significance of the study can help secure funding and support from stakeholders. Funding agencies and institutions are more likely to invest in research that demonstrates clear value and potential impact.

5. Guides Research Focus

The significance section helps guide the research focus by clearly defining the study’s contributions and goals. This clarity ensures that the research stays on track and aligns with its intended purpose.

6. Enhances Academic Credibility

Demonstrating the significance of a study enhances the researcher’s academic credibility. It shows a deep understanding of the field and the ability to identify and address important research questions.

7. Encourages Further Research

By identifying gaps and suggesting future research directions, the significance section encourages other researchers to build on the study’s findings. This fosters a continuous cycle of inquiry and discovery in the field.

8. Highlights Practical Applications

The significance section highlights practical applications of the research, showing how it can solve real-world problems. This makes the study more appealing to practitioners and policymakers who are interested in practical solutions.

9. Increases Research Impact

A clear and compelling significance section increases the overall impact of the research. It enhances the study’s visibility and recognition, leading to broader dissemination and application of the findings.

10. Supports Academic and Professional Goals

For researchers, writing a strong significance section supports academic and professional goals. It can contribute to career advancement, publication opportunities, and recognition within the academic community.

How to Write the Significance of the Study

Writing the significance of a study involves explaining the importance and impact of your research. This section should clearly articulate why your study matters, how it contributes to the field, and what practical applications it may have. Here’s a step-by-step guide to help you write an effective significance of the study:

Start with the Context

Begin by providing a brief overview of the research context. This sets the stage for understanding the importance of your study. Example : “In today’s digital age, digital literacy has become a critical skill for students. As technology continues to integrate into education, understanding its impact on academic performance is essential.”

Identify the Research Gap

Explain the gap in existing literature or the problem your study aims to address. Highlighting this gap justifies the need for your research. Example: “Despite the growing importance of digital literacy, there is limited empirical evidence on its direct impact on high school students’ academic performance. This study seeks to fill this gap by investigating this relationship.”

Explain the Theoretical Contributions

Discuss how your study will contribute to existing theories or knowledge in the field. This shows the academic value of your research. Example : “The findings of this study will contribute to educational theory by providing new insights into how digital literacy skills influence student learning outcomes. It will expand the current understanding of the role of technology in education.”

Highlight Practical Implications

Describe the practical applications of your research. Explain how the findings can be used in real-world settings. Example : “Practically, the results of this study can inform educators and policymakers about the importance of incorporating digital literacy programs into the curriculum. It will help design more effective teaching strategies that enhance students’ digital competencies.”

Mention the Beneficiaries

Identify who will benefit from your study. This could include scholars, practitioners, policymakers, or specific groups affected by the research problem. Example: “This research will benefit educators, school administrators, and policymakers by providing evidence-based recommendations for integrating digital literacy into educational practices. Additionally, students will benefit from improved learning outcomes and better preparedness for the digital world.”

Suggest Future Research

Point out areas for future research that stem from your study. This shows the ongoing relevance and potential for further inquiry. Example : “Future research could explore the long-term effects of digital literacy on career readiness and job performance. Additionally, studies could examine the impact of specific digital literacy interventions on diverse student populations.”

Use Clear and Concise Language

Ensure your writing is clear and concise. Avoid jargon and overly complex sentences to make your significance easily understandable.

What is the significance of a study?

The significance explains the importance, contributions, and impact of the research, highlighting why the study is necessary and how it benefits the field and society.

Why is the significance of a study important?

It justifies the research, engages readers, secures funding, guides the research focus, and highlights practical and theoretical contributions, enhancing the study’s impact and visibility.

How do you identify the significance of a study?

Identify gaps in existing literature, potential contributions to theory and practice, and practical applications that address real-world problems, demonstrating the study’s relevance and importance.

What should be included in the significance of a study?

Include the research context, identified gaps, theoretical contributions, practical applications, beneficiaries, and suggestions for future research to comprehensively explain the study’s importance.

How long should the significance of a study be?

Typically, the significance section should be concise, around 1-2 paragraphs, providing enough detail to clearly convey the study’s importance and contributions.

Can the significance of a study influence funding decisions?

Yes, a well-articulated significance section can attract funding by demonstrating the study’s potential impact and relevance to funding agencies and stakeholders.

How does the significance of a study benefit researchers?

It clarifies the research focus, enhances credibility, guides the research process, and supports academic and professional goals by highlighting the study’s contributions and importance.

Should the significance of a study mention future research?

Yes, mentioning future research directions shows the ongoing relevance of the study and encourages further inquiry, contributing to continuous advancement in the field.

How does the significance of a study relate to the research problem?

The significance justifies the research problem by explaining its importance, highlighting gaps in existing knowledge, and showing how the study addresses these issues.

Can practical applications be part of the significance of a study?

Yes, practical applications are crucial, showing how the research can solve real-world problems, influence practices, and benefit specific groups or society overall.

Text prompt

• Instructive
• Professional

10 Examples of Public speaking

20 Examples of Gas lighting

How To Write a Significance Statement for Your Research

A significance statement is an essential part of a research paper. It explains the importance and relevance of the study to the academic community and the world at large. To write a compelling significance statement, identify the research problem, explain why it is significant, provide evidence of its importance, and highlight its potential impact on future research, policy, or practice. A well-crafted significance statement should effectively communicate the value of the research to readers and help them understand why it matters.

Updated on May 4, 2023

A significance statement is a clearly stated, non-technical paragraph that explains why your research matters. It’s central in making the public aware of and gaining support for your research.

Write it in jargon-free language that a reader from any field can understand. Well-crafted, easily readable significance statements can improve your chances for citation and impact and make it easier for readers outside your field to find and understand your work.

Read on for more details on what a significance statement is, how it can enhance the impact of your research, and, of course, how to write one.

What is a significance statement in research?

A significance statement answers the question: How will your research advance scientific knowledge and impact society at large (as well as specific populations)? 

You might also see it called a “Significance of the study” statement. Some professional organizations in the STEM sciences and social sciences now recommended that journals in their disciplines make such statements a standard feature of each published article. Funding agencies also consider “significance” a key criterion for their awards.

Read some examples of significance statements from the Proceedings of the National Academy of Sciences (PNAS) here .

Depending upon the specific journal or funding agency’s requirements, your statement may be around 100 words and answer these questions:

1. What’s the purpose of this research?

2. What are its key findings?

3. Why do they matter?

4. Who benefits from the research results?

Readers will want to know: “What is interesting or important about this research?” Keep asking yourself that question.

Where to place the significance statement in your manuscript

Most journals ask you to place the significance statement before or after the abstract, so check with each journal’s guide. 

This article is focused on the formal significance statement, even though you’ll naturally highlight your project’s significance elsewhere in your manuscript. (In the introduction, you’ll set out your research aims, and in the conclusion, you’ll explain the potential applications of your research and recommend areas for future research. You’re building an overall case for the value of your work.)

Developing the significance statement

The main steps in planning and developing your statement are to assess the gaps to which your study contributes, and then define your work’s implications and impact.

Identify what gaps your study fills and what it contributes

Your literature review was a big part of how you planned your study. To develop your research aims and objectives, you identified gaps or unanswered questions in the preceding research and designed your study to address them.

Go back to that lit review and look at those gaps again. Review your research proposal to refresh your memory. Ask:

• How have my research findings advanced knowledge or provided notable new insights?
• How has my research helped to prove (or disprove) a hypothesis or answer a research question?
• Why are those results important?

Consider your study’s potential impact at two levels: 

• What contribution does my research make to my field?
• How does it specifically contribute to knowledge; that is, who will benefit the most from it?

Define the implications and potential impact

As you make notes, keep the reasons in mind for why you are writing this statement. Whom will it impact, and why?

The first audience for your significance statement will be journal reviewers when you submit your article for publishing. Many journals require one for manuscript submissions. Study the author’s guide of your desired journal to see its criteria ( here’s an example ). Peer reviewers who can clearly understand the value of your research will be more likely to recommend publication. 

Second, when you apply for funding, your significance statement will help justify why your research deserves a grant from a funding agency . The U.S. National Institutes of Health (NIH), for example, wants to see that a project will “exert a sustained, powerful influence on the research field(s) involved.” Clear, simple language is always valuable because not all reviewers will be specialists in your field.

Third, this concise statement about your study’s importance can affect how potential readers engage with your work. Science journalists and interested readers can promote and spread your work, enhancing your reputation and influence. Help them understand your work.

You’re now ready to express the importance of your research clearly and concisely. Time to start writing.

How to write a significance statement: Key elements 

When drafting your statement, focus on both the content and writing style.

• In terms of content, emphasize the importance, timeliness, and relevance of your research results. 
• Write the statement in plain, clear language rather than scientific or technical jargon. Your audience will include not just your fellow scientists but also non-specialists like journalists, funding reviewers, and members of the public. 

Follow the process we outline below to build a solid, well-crafted, and informative statement. 

Get started

Some suggested opening lines to help you get started might be:

• The implications of this study are… 
• Building upon previous contributions, our study moves the field forward because…
• Our study furthers previous understanding about…

Alternatively, you may start with a statement about the phenomenon you’re studying, leading to the problem statement.

Include these components

Next, draft some sentences that include the following elements. A good example, which we’ll use here, is a significance statement by Rogers et al. (2022) published in the Journal of Climate .

1. Briefly situate your research study in its larger context . Start by introducing the topic, leading to a problem statement. Here’s an example:

‘Heatwaves pose a major threat to human health, ecosystems, and human systems.”

2. State the research problem.

“Simultaneous heatwaves affecting multiple regions can exacerbate such threats. For example, multiple food-producing regions simultaneously undergoing heat-related crop damage could drive global food shortages.”

3. Tell what your study does to address it.

“We assess recent changes in the occurrence of simultaneous large heatwaves.”

4. Provide brief but powerful evidence to support the claims your statement is making , Use quantifiable terms rather than vague ones (e.g., instead of “This phenomenon is happening now more than ever,” see below how Rogers et al. (2022) explained it). This evidence intensifies and illustrates the problem more vividly:

“Such simultaneous heatwaves are 7 times more likely now than 40 years ago. They are also hotter and affect a larger area. Their increasing occurrence is mainly driven by warming baseline temperatures due to global heating, but changes in weather patterns contribute to disproportionate increases over parts of Europe, the eastern United States, and Asia.

5. Relate your study’s impact to the broader context , starting with its general significance to society—then, when possible, move to the particular as you name specific applications of your research findings. (Our example lacks this second level of application.) 

“Better understanding the drivers of weather pattern changes is therefore important for understanding future concurrent heatwave characteristics and their impacts.”

Refine your English

Don’t understate or overstate your findings – just make clear what your study contributes. When you have all the elements in place, review your draft to simplify and polish your language. Even better, get an expert AJE edit . Be sure to use “plain” language rather than academic jargon.

• Avoid acronyms, scientific jargon, and technical terms 
• Use active verbs in your sentence structure rather than passive voice (e.g., instead of “It was found that...”, use “We found...”)
• Make sentence structures short, easy to understand – readable
• Try to address only one idea in each sentence and keep sentences within 25 words (15 words is even better)
• Eliminate nonessential words and phrases (“fluff” and wordiness)

Enhance your significance statement’s impact

Always take time to review your draft multiple times. Make sure that you:

• Keep your language focused
• Provide evidence to support your claims
• Relate the significance to the broader research context in your field

After revising your significance statement, request feedback from a reading mentor about how to make it even clearer. If you’re not a native English speaker, seek help from a native-English-speaking colleague or use an editing service like AJE to make sure your work is at a native level.

Understanding the significance of your study

Your readers may have much less interest than you do in the specific details of your research methods and measures. Many readers will scan your article to learn how your findings might apply to them and their own research. 

Different types of significance

Your findings may have different types of significance, relevant to different populations or fields of study for different reasons. You can emphasize your work’s statistical, clinical, or practical significance. Editors or reviewers in the social sciences might also evaluate your work’s social or political significance.

Statistical significance means that the results are unlikely to have occurred randomly. Instead, it implies a true cause-and-effect relationship.

Clinical significance means that your findings are applicable for treating patients and improving quality of life.

Practical significance is when your research outcomes are meaningful to society at large, in the “real world.” Practical significance is usually measured by the study’s  effect size . Similarly, evaluators may attribute social or political significance to research that addresses “real and immediate” social problems.

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What is the Significance of the Study?

• By DiscoverPhDs
• August 25, 2020

• what the significance of the study means,
• why it’s important to include in your research work,
• where you would include it in your paper, thesis or dissertation,
• how you write one
• and finally an example of a well written section about the significance of the study.

What does Significance of the Study mean?

The significance of the study is a written statement that explains why your research was needed. It’s a justification of the importance of your work and impact it has on your research field, it’s contribution to new knowledge and how others will benefit from it.

Why is the Significance of the Study important?

The significance of the study, also known as the rationale of the study, is important to convey to the reader why the research work was important. This may be an academic reviewer assessing your manuscript under peer-review, an examiner reading your PhD thesis, a funder reading your grant application or another research group reading your published journal paper. Your academic writing should make clear to the reader what the significance of the research that you performed was, the contribution you made and the benefits of it.

How do you write the Significance of the Study?

When writing this section, first think about where the gaps in knowledge are in your research field. What are the areas that are poorly understood with little or no previously published literature? Or what topics have others previously published on that still require further work. This is often referred to as the problem statement.

The introduction section within the significance of the study should include you writing the problem statement and explaining to the reader where the gap in literature is.

Then think about the significance of your research and thesis study from two perspectives: (1) what is the general contribution of your research on your field and (2) what specific contribution have you made to the knowledge and who does this benefit the most.

For example, the gap in knowledge may be that the benefits of dumbbell exercises for patients recovering from a broken arm are not fully understood. You may have performed a study investigating the impact of dumbbell training in patients with fractures versus those that did not perform dumbbell exercises and shown there to be a benefit in their use. The broad significance of the study would be the improvement in the understanding of effective physiotherapy methods. Your specific contribution has been to show a significant improvement in the rate of recovery in patients with broken arms when performing certain dumbbell exercise routines.

This statement should be no more than 500 words in length when written for a thesis. Within a research paper, the statement should be shorter and around 200 words at most.

Significance of the Study: An example

Building on the above hypothetical academic study, the following is an example of a full statement of the significance of the study for you to consider when writing your own. Keep in mind though that there’s no single way of writing the perfect significance statement and it may well depend on the subject area and the study content.

Here’s another example to help demonstrate how a significance of the study can also be applied to non-technical fields:

The significance of this research lies in its potential to inform clinical practices and patient counseling. By understanding the psychological outcomes associated with non-surgical facial aesthetics, practitioners can better guide their patients in making informed decisions about their treatment plans. Additionally, this study contributes to the body of academic knowledge by providing empirical evidence on the effects of these cosmetic procedures, which have been largely anecdotal up to this point.

The statement of the significance of the study is used by students and researchers in academic writing to convey the importance of the research performed; this section is written at the end of the introduction and should describe the specific contribution made and who it benefits.

Statistical treatment of data is essential for all researchers, regardless of whether you’re a biologist, computer scientist or psychologist, but what exactly is it?

The unit of analysis refers to the main parameter that you’re investigating in your research project or study.

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The term research instrument refers to any tool that you may use to collect, measure and analyse research data.

A science investigatory project is a science-based research project or study that is performed by school children in a classroom, exhibition or science fair.

Dr Morrow gained his MD-PhD in Neuroscience from the University of Michigan. He now splits his time between providing clinical care to patients through the University of Michigan and research relevant to addiction and several other psychiatric disorders.

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Home » Feature » Thesis » Significance of the Study Samples | Writing Tips

Significance of the Study Samples | Writing Tips

When you write a thesis , there is a section there that is allocated for the significance of the study. This article will provide different  significance of the study examples and will discuss tips on how to write this part.

Tips in Writing the Significance of the Study

Here are the tips that may be helpful when writing the significance of the study. These tips will tell you the basic components expected to be seen in the significance of the study content.

1. Refer to the Problem Statement

In writing the significance of the study, always refer to the statement of the problem. This way, you can clearly define the contribution of your study. To simplify, your research should answer this question, “What are the benefits or advantages of the study based on the statement of the problem?”

Start by explaining the problem that your study aimed to solve. For example, if you conducted a research study on obesity rates among elementary school students, you would start by explaining that obesity is a major health concern in the Philippines and discuss why it is important to find ways to address this issue.

2. Write it from General to Particular

Determine the specific contribution of your thesis study to society as well as to the individual. Write it deductively, starting from general to specific. Start your significance of the study broadly then narrow it out to a specific group or person. This is done by looking into the general contribution of your study, such as its importance to society as a whole, then moving towards its contribution to individuals like yourself as a researcher.

Discuss how your study fills a gap in the literature. If you conducted an experiment on the effects of a certain type of food on children, for example, you might start by explaining that no research has been done on this topic before. This section would also include a discussion about why your study is important.

Your problem statement might help you determine the unique contribution of your research. This can be accomplished by ensuring that the aim of the problem and the study’s objectives are identical. For instance, if your research question is “Is there a significant relationship between the use of Facebook Messenger and the performance of students in English spelling? “, you could write as one of the contributions of your study: “The study will identify common errors in spelling and grammar by Messenger users and recommend its appropriate use in a way that can improve performance in spelling.”

You may also read: How to Make a Conceptual Framework

Significance of the Study Samples

Here are some examples to help you draft your own introduction:

Title: Number of Clinical Internship Hours: A Determinant of Student’s Effectiveness and Skill  Acquisition in the Hospital Area for Velez College Students

Significance of the study.

The results of the study will be of great benefit to the following:

College of Nursing Dean . Data given will provide the dean with information on how the number of duty hours in a week affects the student’s academic and RLE performance. The results will enable the dean to improve the scheduling of RLE and different academic subjects. Data gathered will help the dean initiate collaboration among faculty and chairpersons to help plan the advancement of nursing education in relation to the new curriculum.

Clinical Instructors . The results of the study will help the clinical instructors evaluate the quality of care rendered by the nursing students, academic performance, attitude and skills acquired in relation to the number of hours given in a week. Results would also develop the clinical instructor’s teaching-learning and evaluating strategies in enhancing knowledge, skills and attitude to the students in the time frame given.

Students . This study will provide information regarding which time arrangement is effective: 8-hr of clinical internship from the 5-hr clinical internship with additional academic classes. This study will evaluate the academic performance, the student nurse’s attitude and approach, the skills learned in the clinical area, and the quality of care rendered in the given time frame. Data gathered will also help the students improve both academic and clinical performance.

Velez College . This study will improve the school in the development of nursing education. This study will foster new ways of enhancing knowledge, skills, and attitude, thus preparing globally-competitive nurses in the future. This study will also help in the advancement of school management, clinical leadership, and the teaching-evaluation approach.

Title: The Effectiveness of Isuzu’s Blue Power Technology in Fuel Efficiency of Diesel Engines

The generalization of this study would be a great contribution to the vast knowledge in relation to the brand awareness of Isuzu’s Blue Power Euro 4 Technology. Furthermore, the results of this investigation could be highly significant and beneficial for the following:

Current Customers

They refer to consumers that have already bought products from Isuzu. They are considered to be the main beneficiaries of the business. The findings of this study would provide them with adequate information about the product, most especially for those clients that have already bought units with the Blue Power Euro 4 Technology but have no idea of its benefits and advantages.

Potential Customer

They are the consumers that have not yet purchased this brand. This study aims to give them insights and overviews of the product and would help them choose the right variant to purchase.

They are the main beneficiaries of this study, which may help them to improve their marketing strategies. It would provide substantial data to the business that they could make use of in boosting their sales. Moreover, developing brand awareness will cater to more demands and loyalty in the future.

For they also play a vital role in the business and as consumers. This research would give them the idea that such private vehicles exist, which helps them to conserve energy rather than exploit it. Hence, giving back to the community and making it a better place to live.

Proponents of the Study

This refers to the students conducting the study. They will find self-fulfillment and gain knowledge and skills in this study. This study will help and inspire more researchers to be more innovative and creative in their future endeavors.

Future Researchers

This study will serve as a reference for researchers on the subject of research in the field of marketing. This will serve as a guide to further developing the research with the connection to the variables used.

The significance of a study is a key component of a strong scientific paper. By following these tips, you can create a clear and concise explanation of the importance of your work. I hope that these tips and samples will help you create a perfect Significance of the Study for your thesis. Apply these tips to prevent your mind from wandering aimlessly as you draft the significance of the study. It will allow you to focus on the next section of your thesis, helping you finish it on time. Good luck!

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Thank you for a very informative article

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How to Write the Rationale of the Study in Research (Examples)

What is the Rationale of the Study?

The rationale of the study is the justification for taking on a given study. It explains the reason the study was conducted or should be conducted. This means the study rationale should explain to the reader or examiner why the study is/was necessary. It is also sometimes called the “purpose” or “justification” of a study. While this is not difficult to grasp in itself, you might wonder how the rationale of the study is different from your research question or from the statement of the problem of your study, and how it fits into the rest of your thesis or research paper. 

The rationale of the study links the background of the study to your specific research question and justifies the need for the latter on the basis of the former. In brief, you first provide and discuss existing data on the topic, and then you tell the reader, based on the background evidence you just presented, where you identified gaps or issues and why you think it is important to address those. The problem statement, lastly, is the formulation of the specific research question you choose to investigate, following logically from your rationale, and the approach you are planning to use to do that.

Table of Contents:

How to write a rationale for a research paper , how do you justify the need for a research study.

• Study Rationale Example: Where Does It Go In Your Paper?

The basis for writing a research rationale is preliminary data or a clear description of an observation. If you are doing basic/theoretical research, then a literature review will help you identify gaps in current knowledge. In applied/practical research, you base your rationale on an existing issue with a certain process (e.g., vaccine proof registration) or practice (e.g., patient treatment) that is well documented and needs to be addressed. By presenting the reader with earlier evidence or observations, you can (and have to) convince them that you are not just repeating what other people have already done or said and that your ideas are not coming out of thin air. 

Once you have explained where you are coming from, you should justify the need for doing additional research–this is essentially the rationale of your study. Finally, when you have convinced the reader of the purpose of your work, you can end your introduction section with the statement of the problem of your research that contains clear aims and objectives and also briefly describes (and justifies) your methodological approach. 

When is the Rationale for Research Written?

The author can present the study rationale both before and after the research is conducted. 

• Before conducting research : The study rationale is a central component of the research proposal . It represents the plan of your work, constructed before the study is actually executed.
• Once research has been conducted : After the study is completed, the rationale is presented in a research article or  PhD dissertation  to explain why you focused on this specific research question. When writing the study rationale for this purpose, the author should link the rationale of the research to the aims and outcomes of the study.

What to Include in the Study Rationale

Although every study rationale is different and discusses different specific elements of a study’s method or approach, there are some elements that should be included to write a good rationale. Make sure to touch on the following:

• A summary of conclusions from your review of the relevant literature
• What is currently unknown (gaps in knowledge)
• Inconclusive or contested results  from previous studies on the same or similar topic
• The necessity to improve or build on previous research, such as to improve methodology or utilize newer techniques and/or technologies

There are different types of limitations that you can use to justify the need for your study. In applied/practical research, the justification for investigating something is always that an existing process/practice has a problem or is not satisfactory. Let’s say, for example, that people in a certain country/city/community commonly complain about hospital care on weekends (not enough staff, not enough attention, no decisions being made), but you looked into it and realized that nobody ever investigated whether these perceived problems are actually based on objective shortages/non-availabilities of care or whether the lower numbers of patients who are treated during weekends are commensurate with the provided services.

In this case, “lack of data” is your justification for digging deeper into the problem. Or, if it is obvious that there is a shortage of staff and provided services on weekends, you could decide to investigate which of the usual procedures are skipped during weekends as a result and what the negative consequences are. 

In basic/theoretical research, lack of knowledge is of course a common and accepted justification for additional research—but make sure that it is not your only motivation. “Nobody has ever done this” is only a convincing reason for a study if you explain to the reader why you think we should know more about this specific phenomenon. If there is earlier research but you think it has limitations, then those can usually be classified into “methodological”, “contextual”, and “conceptual” limitations. To identify such limitations, you can ask specific questions and let those questions guide you when you explain to the reader why your study was necessary:

Methodological limitations

• Did earlier studies try but failed to measure/identify a specific phenomenon?
• Was earlier research based on incorrect conceptualizations of variables?
• Were earlier studies based on questionable operationalizations of key concepts?
• Did earlier studies use questionable or inappropriate research designs?

Contextual limitations

• Have recent changes in the studied problem made previous studies irrelevant?
• Are you studying a new/particular context that previous findings do not apply to?

Conceptual limitations

• Do previous findings only make sense within a specific framework or ideology?

Study Rationale Examples

Let’s look at an example from one of our earlier articles on the statement of the problem to clarify how your rationale fits into your introduction section. This is a very short introduction for a practical research study on the challenges of online learning. Your introduction might be much longer (especially the context/background section), and this example does not contain any sources (which you will have to provide for all claims you make and all earlier studies you cite)—but please pay attention to how the background presentation , rationale, and problem statement blend into each other in a logical way so that the reader can follow and has no reason to question your motivation or the foundation of your research.

Background presentation

Since the beginning of the Covid pandemic, most educational institutions around the world have transitioned to a fully online study model, at least during peak times of infections and social distancing measures. This transition has not been easy and even two years into the pandemic, problems with online teaching and studying persist (reference needed) . 

While the increasing gap between those with access to technology and equipment and those without access has been determined to be one of the main challenges (reference needed) , others claim that online learning offers more opportunities for many students by breaking down barriers of location and distance (reference needed) .  

Rationale of the study

Since teachers and students cannot wait for circumstances to go back to normal, the measures that schools and universities have implemented during the last two years, their advantages and disadvantages, and the impact of those measures on students’ progress, satisfaction, and well-being need to be understood so that improvements can be made and demographics that have been left behind can receive the support they need as soon as possible.

Statement of the problem

To identify what changes in the learning environment were considered the most challenging and how those changes relate to a variety of student outcome measures, we conducted surveys and interviews among teachers and students at ten institutions of higher education in four different major cities, two in the US (New York and Chicago), one in South Korea (Seoul), and one in the UK (London). Responses were analyzed with a focus on different student demographics and how they might have been affected differently by the current situation.

How long is a study rationale?

In a research article bound for journal publication, your rationale should not be longer than a few sentences (no longer than one brief paragraph). A  dissertation or thesis  usually allows for a longer description; depending on the length and nature of your document, this could be up to a couple of paragraphs in length. A completely novel or unconventional approach might warrant a longer and more detailed justification than an approach that slightly deviates from well-established methods and approaches.

Consider Using Professional Academic Editing Services

Now that you know how to write the rationale of the study for a research proposal or paper, you should make use of Wordvice AI’s free AI Grammar Checker , or receive professional academic proofreading services from Wordvice, including research paper editing services and manuscript editing services to polish your submitted research documents.

You can also find many more articles, for example on writing the other parts of your research paper , on choosing a title , or on making sure you understand and adhere to the author instructions before you submit to a journal, on the Wordvice academic resources pages.

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Writing the Significance of a Study

By charlesworth author services.

• Charlesworth Author Services
• 20 July, 2022

The significance of a study is its importance . It refers to the contribution(s) to and impact of the study on a research field. The significance also signals who benefits from the research findings and how.

Purpose of writing the significance of a study

A study’s significance should spark the interest of the reader. Researchers will be able to appreciate your work better when they understand the relevance and its (potential) impact. Peer reviewers also assess the significance of the work, which will influence the decision made (acceptance/rejection) on the manuscript. 

Sections in which the significance of the study is written

Introduction.

In the Introduction of your paper, the significance appears where you talk about the potential importance and impact of the study. It should flow naturally from the problem , aims and objectives, and rationale .

The significance is described in more detail in the concluding paragraph(s) of the Discussion or the dedicated Conclusions section. Here, you put the findings into perspective and outline the contributions of the findings in terms of implications and applications.

The significance may or may not appear in the abstract . When it does, it is written in the concluding lines of the abstract.

Significance vs. other introductory elements of your paper

In the Introduction…

• The problem statement outlines the concern that needs to be addressed.
• The research aim describes the purpose of the study.
• The objectives indicate how that aim will be achieved.
• The rationale explains why you are performing the study.
• The significance tells the reader how the findings affect the topic/broad field. In other words, the significance is about how much the findings matter.

How to write the significance of the study

A good significance statement may be written in different ways. The approach to writing it also depends on the study area. In the arts and humanities , the significance statement might be longer and more descriptive. In applied sciences , it might be more direct.

a. Suggested sequence for writing the significance statement

• Think of the gaps your study is setting out to address.
• Look at your research from general and specific angles in terms of its (potential) contribution .
• Once you have these points ready, start writing them, connecting them to your study as a whole.

b. Some ways to begin your statement(s) of significance

Here are some opening lines to build on:

• The particular significance of this study lies in the… 
• We argue that this study moves the field forward because…
• This study makes some important contributions to…
• Our findings deepen the current understanding about…

c. Don’ts of writing a significance statement

• Don’t make it too long .
• Don’t repeat any information that has been presented in other sections.
• Don’t overstate or exaggerat e the importance; it should match your actual findings.

Example of significance of a study

Note the significance statements highlighted in the following fictional study.

Significance in the Introduction

The effects of Miyawaki forests on local biodiversity in urban housing complexes remain poorly understood. No formal studies on negative impacts on insect activity, populations or diversity have been undertaken thus far. In this study, we compared the effects that Miyawaki forests in urban dwellings have on local pollinator activity. The findings of this study will help improve the design of this afforestation technique in a way that balances local fauna, particularly pollinators, which are highly sensitive to microclimatic changes.

Significance in the Conclusion

[…] The findings provide valuable insights for guiding and informing Miyawaki afforestation in urban dwellings. We demonstrate that urban planning and landscaping policies need to consider potential declines.

A study’s significance usually appears at the end of the Introduction and in the Conclusion to describe the importance of the research findings. A strong and clear significance statement will pique the interest of readers, as well as that of relevant stakeholders.

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How to Write Significance of the Study in a Project Research Paper

When you write your thesis or research paper, there is a section of your introduction that is allotted to the significance of the study. The purpose of this section is to state why your study was needed and the contribution of your research to your field.

In this guide, you will learn the meaning of the significance of the study in your research paper and how to write one.

What is the Significance of the Study?

The significance of the study is basically a written statement that explains why your research was important. It justifies why your research was needed, the impact of your research in your field, its contribution, and how others (audience) would benefit from it.

Also referred to as the rationale of the study, the significance of the study is important to communicate why your research is important to your reader. It is important to make clear the significance of your study for easy comprehension by the readers.

Tips for writing the significance of the study

Reflect on the Problem Statement When writing this section of your paper, first reflect on what contribution your research is making to your field, the gaps in knowledge in your research field, and why your work should be published.

Your problem statement should be reflected in the introduction of the significance of the study. Your research problem statement can guide you to identify specific contributions your research is making to your field of study.

Write from a general contribution to a specific contribution Write your significance of the study in an inverted pyramid format. Start with your research contribution to society as a whole, and then proceed to narrow it down to a specific individual or group of people.

When writing your statement of study, the length should not be more than 500 words for a thesis and around 200 words for a research paper.

However, note that writing the significance of study depends on your subject area and your content as there is no single way of writing a perfect significance of study.

An Example of Significance of Study

This study's findings will further reveal how management-employee bilateral relationships can be strengthened while improving workplace productivity. The findings would be of major importance in assessing how collective bargaining can be a major tool in improving workplace performance in a developing economy like Nigeria. Collective bargaining would help both management and employees bargain on terms and conditions of service and resolve their grievances without leading to strikes, lock-outs, and other forms of industrial actions. The best approach in negotiating on the bargaining table is to provide employees with the importance of collective bargaining as the best method of settling the industrial conflict, which will improve their productivity and lead to higher organizational performance.

The significance of the study is used in academic writing by students and researchers to communicate the importance of a research problem. This section describes specific contributions made to your field of study and who benefits from it. Also, the extent to which the study matters and its potential benefits to people, researchers, departments and other fields are discussed here. This statement is written at the end of your introduction and should be well attended to.

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How to Write an “Implications of Research” Section

4-minute read

• 24th October 2022

When writing research papers , theses, journal articles, or dissertations, one cannot ignore the importance of research. You’re not only the writer of your paper but also the researcher ! Moreover, it’s not just about researching your topic, filling your paper with abundant citations, and topping it off with a reference list. You need to dig deep into your research and provide related literature on your topic. You must also discuss the implications of your research.

Interested in learning more about implications of research? Read on! This post will define these implications, why they’re essential, and most importantly, how to write them. If you’re a visual learner, you might enjoy this video .

What Are Implications of Research?

Implications are potential questions from your research that justify further exploration. They state how your research findings could affect policies, theories, and/or practices.

Implications can either be practical or theoretical. The former is the direct impact of your findings on related practices, whereas the latter is the impact on the theories you have chosen in your study.

Example of a practical implication: If you’re researching a teaching method, the implication would be how teachers can use that method based on your findings.

Example of a theoretical implication: You added a new variable to Theory A so that it could cover a broader perspective.

Finally, implications aren’t the same as recommendations, and it’s important to know the difference between them .

Questions you should consider when developing the implications section:

●  What is the significance of your findings?

●  How do the findings of your study fit with or contradict existing research on this topic?

●  Do your results support or challenge existing theories? If they support them, what new information do they contribute? If they challenge them, why do you think that is?

Why Are Implications Important?

You need implications for the following reasons:

● To reflect on what you set out to accomplish in the first place

● To see if there’s a change to the initial perspective, now that you’ve collected the data

● To inform your audience, who might be curious about the impact of your research

How to Write an Implications Section

Usually, you write your research implications in the discussion section of your paper. This is the section before the conclusion when you discuss all the hard work you did. Additionally, you’ll write the implications section before making recommendations for future research.

Implications should begin with what you discovered in your study, which differs from what previous studies found, and then you can discuss the implications of your findings.

Your implications need to be specific, meaning you should show the exact contributions of your research and why they’re essential. They should also begin with a specific sentence structure.

Examples of starting implication sentences:

●  These results build on existing evidence of…

●  These findings suggest that…

●  These results should be considered when…

●  While previous research has focused on x , these results show that y …

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You should write your implications after you’ve stated the results of your research. In other words, summarize your findings and put them into context.

The result : One study found that young learners enjoy short activities when learning a foreign language.

The implications : This result suggests that foreign language teachers use short activities when teaching young learners, as they positively affect learning.

 Example 2

The result : One study found that people who listen to calming music just before going to bed sleep better than those who watch TV.

The implications : These findings suggest that listening to calming music aids sleep quality, whereas watching TV does not.

To summarize, remember these key pointers:

●  Implications are the impact of your findings on the field of study.

●  They serve as a reflection of the research you’ve conducted.              

●  They show the specific contributions of your findings and why the audience should care.

●  They can be practical or theoretical.

●  They aren’t the same as recommendations.

●  You write them in the discussion section of the paper.

●  State the results first, and then state their implications.

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Reference Examples

More than 100 reference examples and their corresponding in-text citations are presented in the seventh edition Publication Manual . Examples of the most common works that writers cite are provided on this page; additional examples are available in the Publication Manual .

To find the reference example you need, first select a category (e.g., periodicals) and then choose the appropriate type of work (e.g., journal article ) and follow the relevant example.

When selecting a category, use the webpages and websites category only when a work does not fit better within another category. For example, a report from a government website would use the reports category, whereas a page on a government website that is not a report or other work would use the webpages and websites category.

Also note that print and electronic references are largely the same. For example, to cite both print books and ebooks, use the books and reference works category and then choose the appropriate type of work (i.e., book ) and follow the relevant example (e.g., whole authored book ).

Examples on these pages illustrate the details of reference formats. We make every attempt to show examples that are in keeping with APA Style’s guiding principles of inclusivity and bias-free language. These examples are presented out of context only to demonstrate formatting issues (e.g., which elements to italicize, where punctuation is needed, placement of parentheses). References, including these examples, are not inherently endorsements for the ideas or content of the works themselves. An author may cite a work to support a statement or an idea, to critique that work, or for many other reasons. For more examples, see our sample papers .

Reference examples are covered in the seventh edition APA Style manuals in the Publication Manual Chapter 10 and the Concise Guide Chapter 10

Related handouts

• Common Reference Examples Guide (PDF, 147KB)
• Reference Quick Guide (PDF, 225KB)

Textual Works

Textual works are covered in Sections 10.1–10.8 of the Publication Manual . The most common categories and examples are presented here. For the reviews of other works category, see Section 10.7.

• Journal Article References
• Magazine Article References
• Newspaper Article References
• Blog Post and Blog Comment References
• UpToDate Article References
• Book/Ebook References
• Diagnostic Manual References
• Children’s Book or Other Illustrated Book References
• Classroom Course Pack Material References
• Religious Work References
• Chapter in an Edited Book/Ebook References
• Dictionary Entry References
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• Report by a Government Agency References
• Report with Individual Authors References
• Brochure References
• Ethics Code References
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• Press Release References
• White Paper References
• Conference Presentation References
• Conference Proceeding References
• Published Dissertation or Thesis References
• Unpublished Dissertation or Thesis References
• ERIC Database References
• Preprint Article References

Data and Assessments

Data sets are covered in Section 10.9 of the Publication Manual . For the software and tests categories, see Sections 10.10 and 10.11.

• Data Set References
• Toolbox References

Audiovisual Media

Audiovisual media are covered in Sections 10.12–10.14 of the Publication Manual . The most common examples are presented together here. In the manual, these examples and more are separated into categories for audiovisual, audio, and visual media.

• Artwork References
• Clip Art or Stock Image References
• Film and Television References
• Musical Score References
• Online Course or MOOC References
• Podcast References
• PowerPoint Slide or Lecture Note References
• Radio Broadcast References
• TED Talk References
• Transcript of an Audiovisual Work References
• YouTube Video References

Online Media

Online media are covered in Sections 10.15 and 10.16 of the Publication Manual . Please note that blog posts are part of the periodicals category.

• Facebook References
• Instagram References
• LinkedIn References
• Online Forum (e.g., Reddit) References
• TikTok References
• X References
• Webpage on a Website References
• Clinical Practice References
• Open Educational Resource References
• Whole Website References

• Open access
• Published: 12 June 2024

Identifying therapeutic target genes for migraine by systematic druggable genome-wide Mendelian randomization

• Chengcheng Zhang 1 ,
• Yiwei He 2 &

The Journal of Headache and Pain volume  25 , Article number:  100 ( 2024 ) Cite this article

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Currently, the treatment and prevention of migraine remain highly challenging. Mendelian randomization (MR) has been widely used to explore novel therapeutic targets. Therefore, we performed a systematic druggable genome-wide MR to explore the potential therapeutic targets for migraine.

We obtained data on druggable genes and screened for genes within brain expression quantitative trait locis (eQTLs) and blood eQTLs, which were then subjected to two-sample MR analysis and colocalization analysis with migraine genome-wide association studies data to identify genes highly associated with migraine. In addition, phenome-wide research, enrichment analysis, protein network construction, drug prediction, and molecular docking were performed to provide valuable guidance for the development of more effective and targeted therapeutic drugs.

We identified 21 druggable genes significantly associated with migraine (BRPF3, CBFB, CDK4, CHD4, DDIT4, EP300, EPHA5, FGFRL1, FXN, HMGCR, HVCN1, KCNK5, MRGPRE, NLGN2, NR1D1, PLXNB1, TGFB1, TGFB3, THRA, TLN1 and TP53), two of which were significant in both blood and brain (HMGCR and TGFB3). The results of phenome-wide research showed that HMGCR was highly correlated with low-density lipoprotein, and TGFB3 was primarily associated with insulin-like growth factor 1 levels.

Conclusions

This study utilized MR and colocalization analysis to identify 21 potential drug targets for migraine, two of which were significant in both blood and brain. These findings provide promising leads for more effective migraine treatments, potentially reducing drug development costs.

Peer Review reports

Migraine is a prevalent chronic disease characterized by recurring headaches that are typically unilateral and throbbing, ranging from moderate to severe intensity, and often accompanied by nausea, vomiting, sensitivity to light, among other symptoms [ 1 ]. Migraine is recognized as the second most disabling condition globally, creating substantial challenges for those affected and also placing a considerable strain on society overall [ 2 ]. Genetic factors play a substantial role in migraine, with its heritability estimated to be as high as 57% [ 3 ].

Currently, the treatment and prevention of migraine remain highly challenging. Although new drugs (e.g. targeting the calcitonin gene-related peptide, namely CGRP) have been developed, offering significant benefits to migraine sufferers, there are still many issues, such as side effects and less than ideal response rates [ 4 ]. Therefore, it is necessary to continue exploring potential therapeutic targets for migraine treatment. Integrating genetics into drug development may provide a novel approach. While genome-wide association studies (GWAS) are very effective in identifying single nucleotide polymorphisms (SNPs) associated with the risk of migraine [ 5 ], the GWAS method does not clearly and directly identify the causative genes or drive drug development without substantial downstream analyses [ 6 , 7 ].

Mendelian randomization (MR) is a method that utilizes genetic variation as instrumental variables (IVs) to uncover a causal connection between an exposure and an outcome [ 8 ]. MR analysis has been widely applied to discover new therapeutic targets by integrating summarized data from disease GWAS and expression quantitative trait loci (eQTL) studies [ 9 ]. The eQTLs found in the genomic regions of druggable genes are always considered as proxies, since the expression levels of gene can be seen as a form of lifelong exposure. Therefore, we performed a systematic druggable genome-wide MR to explore the potential therapeutic targets for migraine. First, we obtained data on druggable genes and screened for genes within brain eQTLs and blood eQTLs, which were then subjected to two-sample MR analysis with migraine GWAS data to identify genes highly associated with migraine. Subsequently, we conducted colocalization analysis to ensure the robustness of our results. For significant genes both in blood and brain, the phenome-wide research was conducted to explore the relationship between shared potential therapeutic targets and other characteristics. In addition, enrichment analysis, protein network construction, drug prediction, and molecular docking were performed for all significant genes to provide valuable guidance for the development of more effective and targeted therapeutic drugs.

The overview of this study is presented in Fig.  1 .

Overview of this study design. DGIdb: Drug-Gene Interaction Database; eQTL: expression quantitative trait loci; GWAS: genome-wide association studies; PheWAS: Phenome-wide association study; PPI: protein–protein interaction; DSigDB: Drug Signatures Database

Druggable genes

Druggable genes were sourced from the Drug-Gene Interaction Database (DGIdb, https://www.dgidb.org/ ) [ 10 ] and a comprehensive review [ 11 ]. The DGIdb offers insights into drug-gene interactions and the potential for druggability. We accessed the 'Categories Data' from DGIdb, which was updated in February 2022. Additionally, we utilized a list of druggable genes provided in a review authored by Finan et al. [ 11 ]. By consolidating druggable genes from two sources, a broader range of druggable genes can be obtained, which have already been applied in previous study [ 12 ].

eQTL datasets

The blood eQTL dataset was sourced from eQTLGen ( https://eqtlgen.org/ ) [ 13 ], which provided cis-eQTLs for 16,987 genes derived from 31,684 blood samples collected from healthy individuals of European ancestry (Table 1 ). We acquired cis-eQTL results that were fully significant (with a false discovery rate (FDR) less than 0.05) along with information on allele frequencies. We obtained the brain eQTL data from the PsychENCODE consortia ( http://resource.psychencode.org ) [ 14 ], encompassing 1,387 samples from the prefrontal cortex, primarily of European descent (Table 1 ). We downloaded all significant eQTLs (with FDR less than 0.05) for genes that exhibited an expression level greater than 0.1 fragments per kilobase per million mapped fragments in at least 10 samples, along with complete SNP information.

Migraine GWAS dataset

In this study, the summary statistics data for migraine were obtained from a meta-analysis of GWAS conducted by the International Headache Genetics Consortium (IHGC) in 2022 [ 5 ]. To address privacy concerns related to participants in the 23andMe cohort, the GWAS summary statistics data used in this study did not include samples from the 23andMe cohort. The summary data comprised 589,356 individuals of European ancestry, with 48,975 cases and 540,381 controls (Table  1 ).

Mendelian randomization analysis

MR analyses were conducted using the 'TwoSampleMR' package (version 0.5.7) [ 15 ] in R. We chose the eQTLs of the drug genome as the exposure data. For constructing IVs, SNPs with a FDR below 0.05 and located within ± 100 kb of the transcriptional start site (TSS) of each gene were selected. These SNPs were subsequently clumped at an r 2 less than 0.001 using European samples from the 1000 Genomes Project [ 16 ]. The R package 'phenoscanner' [ 17 ] (version 1.0) was employed to identify phenotypes related to the IVs. Additionally, we excluded SNPs that were directly associated with migraine and the trait directly linked to migraine, namely headache. We harmonised and conducted MR analyses on the filtered SNPs. When only one SNP was available for analysis, we use the Wald ratio method to perform MR estimation. When multiple SNPs were available, MR analysis was performed using the inverse-variance weighted (IVW) method with random effects [ 18 ]. We used Cochran's Q test to assess heterogeneity among the individual causal effects of the SNPs [ 19 ]. Additionally, MR Egger's intercept was utilized to evaluate SNP pleiotropy [ 20 ]. P -values were adjusted by FDR, and 0.05 was considered as the significant threshold. Additionally, we selected target genes associated with commonly used medications for migraine and compared their MR results with those of significantly druggable genes.

Colocalization analysis

Sometimes, a single SNP is located in the regions of two or more genes. In such cases, its impact on a disease (here, migraine) is influenced by a mix of different genes. Colocalization analysis was used to confirm the potential shared causal genetic variations in physical location between migraine and eQTLs. We separately filtered SNPs located within ± 100 kb from each migraine risk gene's TSS from migraine GWAS data, blood eQTL data, and brain eQTL data. The probability that a given SNP is associated with migraine is denoted as P1, the probability that a given SNP is a significant eQTL is denoted as P2, and the probability that a given SNP is both associated with migraine and is an eQTL result is denoted as P12. All probabilities were set to default values (P1 = 1 × 10 −4 , P2 = 1 × 10 −4 , and P12 = 1 × 10 −5 ) [ 21 ]. We used posterior probabilities (PP) to quantify the support for all hypotheses, which are identified as PPH0 through PPH4: PPH0, not associated with any trait; PPH1, related to gene expression but not associated with migraine risk; PPH2, associated with migraine risk but not related to gene expression; PPH3, associated with both migraine risk and gene expression, with clear causal variation; and PPH4, associated with both migraine risk and gene expression, with a common causal variant. Given the limited capacity of colocalization analysis, we restricted our subsequent analyses to genes where PPH4 was greater than or equal to 0.75. Colocalization analysis was conducted using the R package 'coloc' (version 5.2.3).

Phenome-wide association analysis

We used the IEU OpenGWAS Project ( https://gwas.mrcieu.ac.uk/phewas/ ) [ 15 ] to obtain the phenome-wide association study (PheWAS) data of SNPs corresponding to druggable genes that were significant in both blood and brain following colocalization analysis.

Enrichment analysis

To explore the functionals' characteristics and biological relevance of predetermined prospective druggable genes, the R package 'clusterProfiler' (version 4.10.1) [ 22 ] was used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment studies. GO includes three terms: Biological Process (BP), Molecular Function (MF), and Cellular Component (CC). KEGG pathways can provide information about metabolic pathways.

Protein–protein interaction network construction

The protein–protein interaction (PPI) networks can visually display the relationships between protein interactions of significant druggable genes. We constructed PPI networks using the STRING ( https://string-db.org/ ) s' with a confidence score threshold of 0.4 as the minimum required interaction score, while all other parameters were maintained at their default settings [ 23 ].

Candidate drug prediction

Drug Signatures Database (DSigDB, http://dsigdb.tanlab.org/DSigDBv1.0/ ) [ 24 ] is a sizable database with 22,527 gene sets and 17,389 unique compounds spanning 19,531 genes. We uploaded previously identified significant druggable genes to DSigDB to predict candidate drugs and evaluate the pharmacological activity of target genes.

Molecular docking

We conducted molecular docking to assess the binding energies and interaction patterns between candidate drugs and their targets. By identifying ligands that exhibit high binding affinity and beneficial interaction patterns, we are able to prioritize drug targets for additional experimental validation and refine the design of prospective candidate drugs. Drug structural data were sourced from the PubChem Compound Database ( https://pubchem.ncbi.nlm.nih.gov/ ) [ 25 ] and downloaded in SDF format, then converted to pdb format using OpenBabel 2.4.1. Protein structural data were downloaded from the Protein Data Bank (PDB, http://www.rcsb.org/ ). The top five important drugs and the proteins encoded by the respective target genes were subjected to molecular docking using the computerized protein–ligand docking software AutoDock 4.2.6 ( http://autodock.scripps.edu/ ) [ 26 ], and the results were visualized using PyMol 3.0.2 ( https://www.pymol.org/ ). The final structures of six proteins and four drugs were obtained.

Druggable genome

We obtained 3,953 druggable genes from the DGIdb (Table S1). Additionally, we acquired 4,463 druggable genes from previous reviews (Table S2) [ 11 ]. After integrating the data, we obtained 5,883 unique druggable genes named by the Human Genome Organisation Gene Nomenclature Committee for subsequent analysis (Table S3).

Candidate druggable genes

After intersecting eQTLs from blood and brain tissue with druggable genes respectively, the blood eQTLs contained 3,460 gene symbols, while the brain eQTLs had 2,624 gene symbols. We performed MR analysis and identified 24 significant genes associated with migraine from blood and 10 from brain tissue (Figs. 2 and 3 ). Among them, two genes, HMGCR and TGFB3, reached significance in both blood (HMGCR OR 1.38 and TGFB3 OR 0.88) and brain tissues (HMGCR OR 2.02 and TGFB3 OR 0.73). Detailed results for the significant IVs and full results of MR are available in the Table S4-S6.

Forest plot of 24 significant genes associated with migraine from blood

Forest plot of 10 significant genes associated with migraine from brain

We selected target genes associated with commonly used medications for migraine as comparisons for our study results [ 27 ]. These include CGRP-related gene (CALCB, CALCRL, RAMP1 and RAMP3), genes related to 5-hydroxytryptamine (5-HT) receptors targeted by ergot alkaloids, triptans, and ditans (HTR1B, HTR1D, HTR1F), γ-aminobutyric acid (GABA) receptor-related genes targeted by topiramate (GABRA1), calcium ion channel-related genes targeted by flunarizine (CACNA1H, CACNA1I, CALM1), and genes related to β-adrenoceptor targeted by propranolol (ADRB1, ADRB2). Among these genes (Fig.  4 ), CALM1 showed significant association with migraine in blood eQTL, but it lost significance after FDR correction (OR 0.92, P  = 0.039, FDR-P = 0.455). In brain eQTL, CALCB and RAMP3 showed correlation with migraine, and after FDR correction, CALCB still maintained significance (CALCB: OR 0.68, P  = 0.0001, FDR-P = 0.029; RAMP3: OR 1.16, P  = 0.031, FDR-P = 0.425).

Forest plot of 13 genes associated with commonly used medications for migraine from blood and brain

The results indicated that, of the previously identified 24 significant genes from blood, 17 had a PPH4 greater than 0.75. Among the 10 significant genes from brain, 6 had a PPH4 greater than 0.75. HMGCR and TGFB3 showed significant colocalization results in both blood and brain tissues (Table  2 , Table  3 and Table S7).

Due to the presence of the blood–brain barrier, compared to various components in the blood and other organs, brain tissue is more difficult to be affected by the action of drugs [ 28 ]. Therefore, we used the IEU OpenGWAS Project to obtain the PheWAS results of SNPs corresponding to HMGCR and TGFB3 from blood, rather than from brain tissue. The results showed that HMGCR was highly correlated with low-density lipoprotein (LDL), and TGFB3 was primarily associated with the level of insulin-like growth factor 1 (IGF1). The complete results are available in the Table S8-S9.

Through GO analysis of 21 potential targets, we found that these targets are primarily involved in BP such as regulation of protein secretion (GO: 0050708), response to hypoxia (GO: 0001666), negative regulation of carbohydrate metabolic processes (GO: 0045912), and the intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator (GO: 0042771). The main MF include transcription coregulator binding (GO: 0001221) and chromatin DNA binding (GO: 0031490, Fig.  5 ). To explore the potential therapeutic pathways of migraine-associated significant druggable genes, KEGG analysis indicates that the target genes were primarily enriched in pathways such as Human T-cell leukemia virus 1 infection (hsa05166) and the Cell cycle (hsa04110, Fig.  6 ).

GO enrichment results for three terms

KEGG enrichment results

We loaded 21 drug target genes into the STRING database to create a PPI network. The results, shown in Fig.  7 , displayed protein interaction pathways consisting of 21 nodes and 22 edges.

PPI network built with STRING

We used DSigDB to predict potentially effective intervention drugs and listed the top 10 potential intervention drugs based on the adjusted P -values (Table  4 ). The results indicated that butyric acid (butyric acid CTD 00007353) and clofibrate (clofibrate CTD 00005684) were the two most significant drugs, connected respectively to TGFB1, TGFB3, EP300, TP53 and TGFB1, CDK4, HMGCR, TP53. Additionally, arsenenous acid (Arsenenous acid CTD 00000922) and dexamethasone (dexamethasone CTD 00005779) were associated with most of the significant druggable genes.

We used AutoDock 4.2.6 to analyze the binding sites and interactions between the top 5 candidate drugs and the proteins encoded by the corresponding genes, generating the binding energy for each interaction. We obtained 14 effective docking results between the proteins and drugs (Table  5 ). Docking amino acid residues and hydrogen bond lengths are shown in Fig. 8 . Among these, the binding between CDK4 and andrographolide exhibited the lowest binding energy (-7.11 kcal/mol), indicating stable binding.

Molecular docking results of available proteins and drugs. a TGFB1 docking butyric acid, b TGFB1 docking clofibrate, c TGFB1 docking Sorafenib, d TGFB1 docking Andrographolide, e TGFB3 docking butyric acid, f EP300 docking butyric acid, g TP53 docking butyric acid, h CDK4 docking clofibrate, i CDK4 docking Sorafenib, j CDK4 docking Andrographolide, k HMGCR docking clofibrate, l TP53 docking clofibrate, m TP53 docking Sorafenib, n TP53 docking Andrographolide

This study integrated existing druggable gene targets with migraine GWAS data through MR and colocalization analysis, identifying 21 druggable genes significantly associated with migraine (BRPF3, CBFB, CDK4, CHD4, DDIT4, EP300, EPHA5, FGFRL1, FXN, HMGCR, HVCN1, KCNK5, MRGPRE, NLGN2, NR1D1, PLXNB1, TGFB1, TGFB3, THRA, TLN1 and TP53). To further illustrate the potential pleiotropy and drug side effects of significant druggable genes, we conducted a phenome-wide research of two SNPs associated with two druggable genes of interest (HMGCR and TGFB3). Additionally, we performed enrichment analysis and constructed PPI network for these 21 significant genes to understand the biological significance and interaction mechanisms of these drug targets. Finally, drug prediction and molecular docking were conducted to further validate the pharmaceutical value of these significant druggable genes.

The association between HMGCR and migraine has been supported by multiple prior studies. One study indicated that migraine has significant shared signals with certain lipoprotein subgroups at the HMGCR locus [ 29 ]. Hong et al. found that HMGCR genotypes associated with higher LDL cholesterol levels are linked to an increased risk of migraine [ 30 ]. Statins inhibit the activity of HMG-CoA reductase, which is encoded by the HMGCR gene, to exert their lipid-lowering effects and have been widely used in the prevention and treatment of coronary heart disease and ischemic stroke. Previous clinical research has shown that simvastatin combined with vitamin D can effectively prevent episodic migraines in adults [ 31 ]. Additionally, HMGCR may also be involved in immune modulation, with studies suggesting that migraine patients experience neuroinflammation due to activation of the trigeminal-vascular system, leading to peripheral and central sensitization of pain and triggering migraine attacks [ 32 , 33 ]. HMGCR inhibitors can suppress the production of inflammatory mediators and cytokines, thus reducing inflammatory responses [ 34 ]. We speculate that the role of HMGCR in regulating inflammation and immunity may have influenced the drug prediction results generated by DSigDB, which based on Gene Set Enrichment Analysis (GSEA) [ 24 , 35 , 36 ], diluting the role of HMGCR in regulating lipid metabolism. Therefore, statins did not appear in the predicted list of candidate drugs.

TGFB1 and TGFB3 encodes different secreted ligands of the transforming growth factor-beta (TGF-β) superfamily of proteins, namely TGF-β1 and TGF-β3. TGF-β is a pleiotropic cytokine closely associated with immunity and inflammation [ 37 ]. Research indicated that TGF-β3 can inhibit B cell proliferation and antibody production by suppressing the phosphorylation of NF-κB, thus exerting its anti-inflammatory effects [ 38 ]. The activation of the classical NF-κB pathway is a key mechanism that upregulates pro-inflammatory cytokines, promoting central sensitization and leading to the onset of chronic migraine [ 39 ]. A previous clinical study indicated that the serum levels of TGF-β1 are significantly elevated in migraine patients [ 40 ]. Ishizaki et al. found that TGF-β1 levels in the platelet poor plasma of migraine patients are significantly increased during headache-free intervals [ 41 ]. Bø et al. discovered that during acute migraine attacks, the levels of TGF-β1 in cerebrospinal fluid are significantly higher compared to the control group [ 42 ]. Although some studies consider TGF-β1 to be an anti-inflammatory cytokine [ 43 ], based on previous research and the results of this study, we believe that TGFB1 and its encoded protein, TGF-β1, are associated with an increased risk of migraine. The pleiotropic effects of TGF-β1 on inflammation may depend on concentration and environment [ 44 ]. In addition, we found an association between TGFB3 and IGF1 in our phenome-wide research. A previous MR study showed that increased levels of IGF1 are causally associated with decreased migraine risk [ 45 ]. Recent experimental results suggest that the miR-653-3p/IGF1 axis regulating the AKT/TRPV1 signaling pathway may be a potential pathogenic mechanism for migraine [ 46 ]. The beneficial effects of TGF-β3 and IGF1 on migraine may be associated with the regulation of gene expression in different microenvironments to promote the transition of microglial cells from M1 (pathogenic) to M2 (protective) phenotypes [ 47 ].

Among the 13 genes targeted by some commonly used migraine treatment drugs, the MR results for 3 genes were significant in blood or brain eQTL. Although only one gene remained significant after FDR correction, this still demonstrates that the significant genes newly identified in this study are reliable and have potential as drug targets to some extent. The lack of significance in certain drug target genes may be related to the insufficient sample size of the migraine GWAS data included in our study. It would be meaningful to validate the results of this study with more large-sample GWAS data available in the future.

In this study, DSigDB predicted 10 potential drugs for migraine, but current clinical research is mainly focused on melatonin and dexamethasone. ClinicalTrials ( https://clinicaltrials.gov/ ) has registered multiple studies on the efficacy of melatonin and dexamethasone for migraine. Many research findings differ differently and controversially. A published clinical study on acute treatment of pediatric migraine showed that both low and high doses of melatonin contributed to pain relief [ 48 ]. The consensus published by the Brazilian Headache Society in 2022 lists melatonin as a recommended medication for preventing episodic migraine (Class II; Level C) [ 49 ]. However, study indicated that bedtime administration of sustained-release melatonin did not lead to a reduction in migraine attack frequency compared to placebo [ 50 ]. Dexamethasone has shown good efficacy for severe acute migraine attacks [ 51 ]. The 2016 guidelines for the emergency treatment of acute migraines in adults, issued by the American Headache Society, mention that dexamethasone should be administered to prevent the recurrence of migraine (Should offer—Level B) [ 52 ]. But study suggested that dexamethasone does not reduce migraine recurrence [ 53 ].

An animal study has shown that clofibrate can improve oxidative stress and neuroinflammation caused by the exaggerated production of lipid peroxidation products [ 54 ]. Clofibrate can activate peroxisome-proliferator-activated receptors (PPAR) α, inhibit the activation of the NF-κB signaling pathway and the production of interleukin (IL)-6, exerting an anti-inflammatory effect [ 55 , 56 ]. Additionally, a recent animal study indicated the upregulation of astrocytic activation and glial fibrillary acidic protein (GFAP) expression in the trigeminal nucleus caudalis (TNC) in migraine mice model induced by recurrent dural infusion of inflammatory soup (IS). This was accompanied by the release of various cytokines, increased neuronal excitability, and promotion of central sensitization processes [ 57 ]. Clofibrate can reduce the activation of astrocytes and the expression of GFAP, thereby inhibiting neuroinflammation [ 54 ]. Andrographolide is a major bioactive constituent of Andrographis paniculata, has broad effects on various inflammatory and neurological disorders [ 58 , 59 , 60 ]. Although we did not find any migraine clinical trials related to clofibrate and andrographolide on PubMed and ClinicalTrials, we believe that the prospects for using clofibrate and andrographolide in the treatment of migraine are quite promising. We hope to see more research on the association of clofibrate and andrographolide with migraine in the future.

Our study has several advantages. First, we provided compelling genetic evidence about migraine drug targets using MR, utilizing the largest publicly available GWAS data to date. Additionally, colocalization analysis helps reduce false negatives and false positives to ensure the robustness of the results. Enrichment analysis and PPI illustrate the functional characteristics and regulatory relationships of these targets genes, providing potential avenues for migraine drug development. The drug predictions demonstrate the medicinal potential of these genes, and high binding activity from molecular docking indicates the strong potential of these genes as drug targets. Our research conducts a comprehensive evaluation from identifying migraine-related druggable genes to drug binding properties, proposing migraine drug targets with compelling evidence.

This study also includes several notable limitations. Firstly, the number of eQTL IVs in MR is limited, with most not exceeding three SNPs, which restricts the credibility of the MR results. Additionally, while MR offers valuable insights into causality, it assumes a linear connection between low-dose drug exposure and the exposure-outcome relationship, which may not fully replicate real-world clinical trials that typically assess high doses of drugs in a short timeframe. Therefore, MR results may not accurately reflect the effect sizes observed in actual clinical settings, nor fully predict the impacts of drugs. Secondly, the generalizability of this study is limited by its primary inclusion of individuals of European descent. Extrapolating the findings to individuals of other genetic ancestry populations requires further research and validation to ensure broader applicability. Thirdly, the study focuses mainly on cis-eQTLs and their relationship with migraine, potentially overlooking other regulatory and environmental factors that contribute to the complexity of the disease. Fourthly, while enrichment analysis is valuable, it has inherent limitations as it relies on predefined gene sets or pathways, which may not encompass all possible biological mechanisms or interactions. A lack of significant enrichment does not necessarily mean there is no biological relevance, and researchers should interpret results cautiously. Fifth, the accuracy of molecular docking analysis largely depends on the quality of the protein structures and ligands. While this method identified potential drug targets, it does not guarantee their efficacy in clinical settings. Subsequent experimental validation and clinical trials are necessary to confirm the therapeutic potential of the identified targets. Moreover, we only investigated the side effects of 2 significant druggable genes. The effects of drugs on targets are very broad, and many off-target effects cannot be explored through MR, requiring further basic and clinical trials to gain a more comprehensive understanding. Finally, the clinical relevance of our study results needs further validation; the lack of clinical data related to our study is a significant limitation.

This study utilized MR and colocalization analysis to identify 21 potential drug targets for migraine, two of which were significant in both blood and brain. These findings provide promising leads for more effective migraine treatments, potentially reducing drug development costs. The study contributes valuably to the field, highlighting the importance of these druggable genes significantly associated with migraine. Further clinical trials on drugs targeting these genes are necessary in the future.

Availability of data and materials

The Migraine GWAS dataset provided by Hautakangas et al. can be obtained by contacting International Headache Genetics Consortium [ 5 ]. Other data can be obtained from the original literature and websites.

Abbreviations

• Mendelian randomization

Expression quantitative trait loci

Genome-wide association studies

Calcitonin gene-related peptide

Single nucleotide polymorphisms

Instrumental variables

Drug-Gene Interaction Database

False discovery rate

International Headache Genetics Consortium

Transcriptional start site

Inverse-variance weighted

5-Hydroxytryptamine

γ-Aminobutyric acid

Posterior probabilities

Phenome-wide association study

Gene Ontology

Kyoto Encyclopedia of Genes and Genomes

Biological process

Molecular function

Cellular component

Protein–protein interaction

Drug Signatures Database

Protein Data Bank

Low-density lipoprotein

Gene Set Enrichment Analysis

Insulin-like growth factor 1

Transforming growth factor-beta

Peroxisome-proliferator-activated receptors

Interleukin

Glial fibrillary acidic protein

Trigeminal nucleus caudalis

Inflammatory soup

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Acknowledgements

The authors sincerely thank related investigators for sharing the statistics included in this study.

This study was funded by China National Natural Science Foundation (82374575, 82074179), Beijing Natural Science Foundation (7232270), Outstanding Young Talents Program of Capital Medial University (B2207), Capital’s Funds for Health Improvement and Research (CFH2024-2–2235).

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LL contributed to the study conception and design. CCZ and YWH performed the statistical analysis. CCZ drafted the manuscript. All authors commented on previous versions of the manuscript. All authors contributed to the article and approved the submitted version.

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Zhang, C., He, Y. & Liu, L. Identifying therapeutic target genes for migraine by systematic druggable genome-wide Mendelian randomization. J Headache Pain 25 , 100 (2024). https://doi.org/10.1186/s10194-024-01805-3

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How does population aging affect green building development?:a study from China evidence

• Published: 20 June 2024

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• Bin Liao   ORCID: orcid.org/0000-0002-7663-6406 1 &
• Lin Li 1 , 2  

Governments of China are vigorously promoting the green building development, aiming to solve the problem of heavy pollution emissions from the traditional construction industry. However, previous studies on green building development have ignored the impact of the trend of global population aging. In order to fill this gap, this paper creatively constructs a theoretical analysis framework of the impact of population aging on the green building development, and then makes an empirical test by using the panel number of 30 provinces in China from 2008 to 2019.The results show that the population aging has an inverted “U” effect on the green building development, in which the consumption demand of the elderly, the labor productivity of the construction industry the aging preference of financial structure play a mediating effect, but the mechanism is different. In addition, there is regional heterogeneity in the impact of population aging on the green building development. at present, only the aging population in the eastern part of China has a significant inverted “U” impact on the green building development. The above research conclusions have certain theoretical and practical significance for government departments to promote the green building development in the coming aging society.

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Acknowledgements

This project is supported by Xiangtan Municipal Social Science Federation 2024 ‘Leadersgive questions-experts answer questions’. Social science planning projects (No. 2024035).

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Liao, B., Li, L. How does population aging affect green building development?:a study from China evidence. Environ Dev Sustain (2024). https://doi.org/10.1007/s10668-024-05139-7

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Women’s techniques for pleasure from anal touch: Results from a U.S. probability sample of women ages 18–93

Roles Formal analysis, Writing – original draft, Writing – review & editing

* E-mail: [email protected]

Affiliations Department of Pediatrics, Division of Adolescent Medicine, Indiana University School of Medicine, Indianapolis, Indiana, United States of America, Department of Sociology, Indiana University Purdue University-Indianapolis, Indianapolis, Indiana, United States of America, OMGYES Research Group, For Goodness Sake LLC, Berkeley, California, United States of America

Roles Writing – original draft, Writing – review & editing

Affiliation OMGYES Research Group, For Goodness Sake LLC, Berkeley, California, United States of America

Roles Conceptualization, Project administration, Writing – original draft, Writing – review & editing

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Writing – original draft, Writing – review & editing

• Devon J. Hensel, 
• Christiana D. von Hippel, 
• Charles C. Lapage, 
• Robert H. Perkins

• Published: June 29, 2022
• https://doi.org/10.1371/journal.pone.0268785
• Reader Comments

The study purpose was to assess, in a U.S. probability sample of women, the specific ways women have discovered to experience pleasure from anal touch. Through qualitative pilot research with women that informed the development of the survey instrument used in this study, we identified three previously unnamed, but distinct, anal touch techniques that many women find pleasurable and that expand the anal sexual repertoire beyond the more commonly studied anal intercourse behaviors: Anal Surfacing, Anal Shallowing, and Anal Pairing. This study defines each technique and describes its prevalence among U.S. adult women. Weighted frequencies were drawn from the Second OMGYES Pleasure Report—a cross-sectional, online, national probability survey of 3017 American women’s (age 18–93) sexual experiences and discoveries. Participants were recruited via the Ipsos KnowledgePanel ® . Data suggest that 40% of women find ‘Anal Surfacing’ pleasurable: sexual touch by a finger, penis, or sex toy on and around the anus. Approximately 35% of women have experienced pleasure using ‘Anal Shallowing’: penetrative touch by a finger, penis, or sex toy just inside the anal opening, no deeper than a fingertip/knuckle. Finally, 40% of women make other forms of sexual touch more pleasurable using ‘Anal Pairing’: touch on or inside the anus that happens at the same time as other kinds of sexual touch such as vaginal penetration or clitoral touching. These data provide techniques that women can and do use to explore the anus as a pleasurable region for touch—which can enable women to better identify their own preferences, communicate about them and advocate for their sexual pleasure.

Citation: Hensel DJ, von Hippel CD, Lapage CC, Perkins RH (2022) Women’s techniques for pleasure from anal touch: Results from a U.S. probability sample of women ages 18–93. PLoS ONE 17(6): e0268785. https://doi.org/10.1371/journal.pone.0268785

Editor: Andrew R. Dalby, University of Westminster, UNITED KINGDOM

Received: November 16, 2021; Accepted: May 4, 2022; Published: June 29, 2022

Copyright: © 2022 Hensel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All raw data files used in this study are available from the Inter-university Consortium for Political and Social Research (ICPSR) open data sharing consortium (“Second OMGYES Pleasure Report – Anal Touch Items”: https://doi.org/10.3886/E136982V1 ).

Funding: CDvH, CCL, and RHP received funding in the form of salary from Goodness Sake, LLC. DJH received funding as a paid consultant for Goodness Sake LLC. RHP received funding in the form of salary from Geno LLC. The funders had a role in study design, data collection and analysis, decision to publish, or and preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.

Competing interests: CDvH, CCL, and RHP were salaried employees of Goodness Sake LLC during the course of the study. DJH was a paid consultant of Goodness Sake LLC during the course of the study. RHP was also a salaried employee of Geno LLC during the course of the study. There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

Introduction

The purpose of this study is to use nationally representative data to describe the ways in which adult women use different anal touch and stimulation techniques–defined for the purposes of this paper as penetration or internal/external stimulation of the anus with a penis, finger(s), lips/tongue, sex toy, or other object [ 1 ]–as a means of achieving sexual pleasure. The geography of the anus makes it a highly pleasurable sex organ: it contains a dense network of sensory nerves that participate with the genitals in the engorgement, muscular tension and contractions of sexual arousal and orgasm [ 2 , 3 ].

Despite this anatomic pleasure potential [ 4 ], much of the existing published scientific research on anal sexuality narrowly focuses on what body part–typically a penis, and less often a mouth/tongue or finger–is stimulating or penetrating the anus, rather than thinking more broadly about how women and their partners may individuate stimulation and penetration techniques. For example, existing studies most commonly operationalize “anal sex” for women in terms of penetration of their anus with a male partner’s penis. The prevalence of women’s participation in anal sex varies depending both upon the behavior as well as the timeframe of reference. Different nationally representative studies from the past decade suggest that about a third of women have lifetime experience with penile-anal sex [ 5 – 10 ]. Between 11.8% and 13.2% have engaged in penile-anal sex in the past year [ 5 , 6 ] and about 10% have done so in the past 90 days [ 11 ]. Less than five percent of women report engaging in anal sex during their most recent sexual experience [ 12 ]. A less developed body of literature using nationally representative data from the United States and from Australia suggests that women have also participated in other anal stimulation and penetration techniques, such as digital-penetration, manual stimulation, and/or oral-anal contact. This work suggests that between five percent and 43% of women have participated in oral-anal contact [ 1 , 13 , 14 ], between 15% and 20% have had a partner digitally stimulate and/or penetrate their anus [ 1 , 15 ] and very few have ever had a partner insert a fist into their anus [ 14 ]. None of this literature assessed the specific touch techniques that were used by women and their partners to stimulate the anus internally or externally, or to what extent they were pleasurable.

A lack of detailed scientific studies on women’s anal pleasure techniques means that women and their partners likely receive more information from adverse-outcome focused peer-reviewed literature or from print/electronic/film media, about what women’s anal sex lives “look” like and/or how they “should” feel about engaging specific practices. In public health literature, for example, anal sex–especially when defined as women’s receipt of penile penetration of their anus–typically broadly quantifies frequency and with whom anal sex practices occur, often framing the outcomes in terms of potential “risks” (e.g. sexually transmitted infections, pain, bodily injury) [ 16 ], rather than emphasizing how women and partners work to choose specific stimulation and penetration techniques that are associated with positive outcomes like pleasure. Peer-reviewed scientific studies do examine women’s experiences with receptive penile-anal sex, and while some do document pleasure as a motivator [ 17 , 18 ], far more emphasize adverse aspects, such as the cultural stigma associated with anal sex, or how women do not enjoy or are coerced into participating in anal sex [ 19 ]. Next to nothing is known about either the ways in which women actively choose different techniques that are not penile-anal penetration or about how pleasure levels may vary across these techniques [ 20 ]. Popular print and electronic media interest in anal sex has increased in the past decade, with common emphasis on the “right way” or “how to”–often using slang or ambiguous terms (e.g. “butt sex”)–to both prepare for and to engage in penile-anal sex [ 21 , 22 ]. These sources rarely acknowledge a wider repertoire of anal stimulation and stimulation methods that women can and do choose [ 23 ].

In the current paper, we use data from the second OMGYES Pleasure Report to address both a measurement and a sampling gap in the literature about women’s experiences with anal stimulation and penetration. The second OMGYES Pleasure Report is a nationally representative study designed to investigate women’s preferences for genital touch/stimulation and penetration, including those involving the anus. From a measurement perspective, it is important for sexual pleasure research to assess a range of ways that women use for anal touch that are not currently explored in existing studies, as it elicits the information needed to both validate what women are doing, and also to provide the techniques names that are accessible and non-ambiguous. Usable terminology scaffolds a woman’s ability to communicate with partners, as well her ability to learn new techniques and/or to tinker with existing techniques she is already using [ 24 ]. We have used this level of specificity in detail and naming in a prior nationally representative study, the first OMGYES pleasure report , which explored women’s experiences with external vulvar and clitoral touch techniques [ 25 ] as well as a previous paper using data from the second OMGYES Pleasure Report describing women’s preferences for internal vaginal touch techniques [ 24 ]. In both these previous studies, rather than asking participants whether they liked to be touched externally or inside their vagina, the data demonstrated that women use different ways of touching–different locations, pressure, shapes, and patterns–as a means of increasing their sexual enjoyment. From a sampling perspective, although existing nationally representative studies in the United States assess women’s participation in penile-anal sex [ 5 – 12 ], the lack of other anal stimulation and penetration techniques means that their prevalence and patterns at the population level are unknown [ 24 ]. Such data are important to be able to reflect the experiences of all women in the United States.

Accordingly, the purpose of the current study was to use nationally representative probability data from the second OMGYES Pleasure Report to investigate women’s experiences and preferences for anal stimulation and penetration.

Materials and methods

Data collection.

Data for the current study were drawn from the second OMGYES Pleasure Report —a cross-sectional, online, nationally representative survey of sexual behaviors, sexual attitudes, relationships, sexual satisfaction, and experiences with genital touching among women aged 18 and over in the United States. The study was conducted in July 2018 by Ipsos Research using their KnowledgePanel ® (Menlo Park, California) to recruit a probability-based web panel designed to be representative–including an oversample of lesbian and bisexual women–of all noninstitutionalized U.S. women. The 90-item online survey took a median of 29 minutes to complete, was available in English, and was open for participation from July 12-July 31, 2018. Questions assessed participation in demographics, sexual behavior background, as well as lifetime participation in different types of Anal Surfacing, Anal Shallowing, and Anal Pairing . All sexual touch technique items are original to this study and have not been yet examined in the peer-reviewed literature. Definitions and sexually explicit line drawing illustrations of these four techniques are provided in an online S1 Table . Images contained in this table are visually graphic.

Of those who opened the study link, 88.8% (3017/3398) completed the survey (49.7% [3017/6123] of the initial sampling frame) and represent the analytical sample in this study. This completion rate is similar to other Ipsos-conducted nationally representative studies of sexuality and sexual behavior (44%–51%) [ 5 , 26 – 28 ]. Post-stratification, study-specific weights adjusted for over- or under-sampling as well as non-response. Participants provided electronic informed consent. All study procedures were approved by the institutional review board at Indiana University School of Medicine (IRB # 1801846511). Additional methodological details–for both this survey as well as the development of this survey–are available in Hensel et al. [ 24 ] All data used in this study are available through the ICPSR open data sharing consortium [ 29 ].

Participants were asked about their experiences with three domains of anal touch and stimulation techniques, including Anal Surfacing, Anal Shallowing , and Anal Pairing . These domains were originally identified as part of qualitative work conducted prior to the larger study (additional detail on this work is available in Hensel et al., 2021: [ 24 ]). Anal Surfacing emerged through a theme of many women’s discovery that anal touch could be pleasurable when the anus was reconceptualized as a flat erogenous zone, which could be stimulated with touch on the surface, as opposed to solely as an opening for penetration. Anal Shallowing emerged from the qualitative analysis through a theme that demonstrated many women had discovered anal pleasure with shallow penetrative touch just inside the anal opening, in contrast to the deeper penetration commonly associated with the concept of anal sex. Anal Pairing emerged from the theme of women’s insight that when anal touch occurs simultaneously with other forms of sexual touch (e.g., vaginal penetration, clitoral touching), it can make the sexual experience more pleasurable. This is consistent with findings from our previous research on techniques women use for pleasure during vaginal penetration where Pairing, in this case of clitoral stimulation with vaginal stimulation, helped 69.7% of women orgasm more often or make vaginal penetration more pleasurable [ 24 ]. Definitions and sexually explicit line art illustrations of all three anal touch techniques are provided in the S1 Table .

Questions about anal touch were prefaced in the survey with a statement that said: “One area of sexuality that’s rarely talked about is anal touch/stimulation, even though nearly half of Americans have tried anal play. We are not talking just about anal penetration or what’s commonly called ‘anal sex.’ Rather, we are talking about any kind of touch of the outside or inside of the anus or butthole with a fingertip, toy, penis or anything else.”

Anal surfacing.

Participants were first asked the extent to which they had ever found different methods of “touch on the outside of your anus/butthole” pleasurable when used either during sex with a partner or alone during masturbation (all: four-point Likert scale: 1. not pleasurable to 4. very pleasurable; or “don’t know or never tried”). The four items related to specific sub-forms of Anal Surfacing were: “with your own finger,” “with your partner’s finger,” “with a sex toy,” and “with a penis.” All items were dichotomized as pleasurable (a little/somewhat/very pleasurable) vs. not (not at all pleasurable/don’t know or never tried) for analysis. A response of a little/somewhat/very pleasurable to at least one anal surfacing item indicated a woman had found any form of anal surfacing pleasurable.

Anal penetration and shallowing.

Participants were asked the extent to which they had ever found different methods of “penetration inside of your anus/butthole (either shallow or deeper)” pleasurable when used either during sex with a partner or alone during masturbation (all: four-point Likert scale: 1. not pleasurable to 4. very pleasurable; or “don’t know or never tried”). The four items related to specific sub-forms of Anal Penetration were: “with your own finger,” “with your partner’s finger,” “with a sex toy,” and “with a penis.” All items were dichotomized as pleasurable (a little/somewhat/very pleasurable) vs. not (not at all pleasurable/don’t know or never tried) for analysis. A response of a little/somewhat/very pleasurable to at least one anal penetration item indicated a woman had found any form of anal penetration pleasurable.

Women who found any form of anal penetration pleasurable were then asked the multiple response item “You mentioned you found penetration inside your anus pleasurable. At what depth have you found anal stimulation most pleasurable?” Participants selected the depth that applied from the following list: “just barely inside of anus/butthole (such as the very tip of a finger);” “about 1-knuckle inside;” “2-knuckles deep or as deep as an entire finger can go;” “deeper inside than an entire finger can usually reach;” or they selected “I don’t know.” A selection of either the “just barely inside of anus/butthole (such as the very tip of a finger)” or the “about 1-knuckle inside” depth response option indicated a woman had found any form of Anal Shallowing pleasurable. A selection of any of the greater depths of penetration as most pleasurable indicated a woman enjoyed deeper anal penetration.

Prior experience and pleasure from any form of anal touch.

A response of a little/somewhat/very pleasurable to at least one Likert scaled anal surfacing or anal penetration (shallow or deeper) item indicated a woman had experienced pleasure with any form of anal touch. Conversely, a response of “I don’t know or never tried” to every Likert scaled anal surfacing and anal penetration (shallow or deeper) item indicated a woman had no prior experience with or knowledge of pleasure from any form of anal touch. Subsequent measures described below were asked only of the subgroup of women with prior experience of pleasure from any form of anal touch. These measures were used to evaluate patterns observed in the qualitative research phase relating to women’s experiences of discovering anal touch could be pleasurable and specific ways in which it was pleasurable for them.

Assessment of anal pleasure discovery patterns.

To discern whether women’s enjoyment of anal touch was immediate or gradual, women who indicated that they found any form of external anal touch pleasurable were asked which statement best described their experience with touch outside their anus: either, “You found it pleasurable from the first time you tried it;” or, “You didn’t find it pleasurable at first, but learned to enjoy it over time.” In the same fashion, women who indicated they found any form of anal penetration pleasurable were asked which of the same two statements as above best described their experience with anal penetration.

Additionally, women who previously indicated they enjoyed any form of anal touch were asked to enter into number boxes the approximate ages in years at which they discovered that touch on the outside of the anus could be pleasurable and/or discovered that touch inside the anus could be pleasurable. If they did not recall their age, women entered “99;” all responses of “99” and women missing responses were excluded from the analyses of age at discovery. Additionally, age 14 years at discovery was used as the lower bound for analysis since this is a typical age at which young people in U.S. states can consent to sexual, reproductive health services without parental permission [ 30 ].

Factors contributing to women’s discovery of pleasure with any form of anal touch were assessed with a multiple response item: “Over time, which of the following do you think made you realize anal stimulation could be pleasurable for you?” Response options were “during self-exploration/masturbation, I found a way of anal stimulation that I liked;” “a partner approached anal sex in a way that worked for me;” “trying anal touch or anal sex on my own terms (e.g., communicating about the way I wanted to do it and having that respected);” “once I/we used enough lubricant;” “I had enough "warm up time" beforehand;” “trying it with someone I loved or deeply cared for;” “a partner just barely touched or brushed by my anus/butthole and I enjoyed it;” “I felt an increased desire for anal sex during or since pregnancy;” “I felt an increased desire for anal sex during or after menopause;” “other.”

Anal pairing and types of pleasure experienced from anal touch.

A single multiple response item asked women about the ways in which anal touch is pleasurable for them: “Other women have suggested the following reasons why anal stimulation is pleasurable. What do you think it is about anal stimulation that makes it pleasurable for you?” Respondents could select one or more of the following response options to describe their experience: “I can have orgasms just from anal touch/stimulation;” “it can make my orgasms feel more intense;” “it can make it easier for me to have an orgasm during other kinds of touch;” “when it happens at the same time as other kinds of touch (like vaginal sex or clitoral touching), it can make the experience more pleasurable;” “It has its own unique sensation that I find pleasurable;” “I get a thrill from the feeling that anal play is taboo;” “it feels profoundly intimate and emotional;” the pleasure feels fuller or ‘bigger’ than other kinds of sexual pleasure;” “other.” The selection of one or both of the two response options relating to pleasure from anal stimulation as an enhancement of their pleasure when done simultaneously with other touch indicated a woman found Anal Pairing pleasurable.

Statistical procedure

Weighted frequencies were calculated to assess the prevalence of women who have used Angling, Rocking, Shallowing, Pairing, and their sub-forms to make vaginal stimulation and penetration more pleasurable. We excluded from analysis of each item any participant whose response to that item was missing. IBM SPSS Statistics software was used for all analyses.

Respondent characteristics

Weighted respondent demographic characteristics—including age, gender, race/ethnicity, education, household income, and geographic region of residence in the US, sexual orientation and relationship status—are presented in Table 1 . Women ranged in age from 18 to 93 with a median age of 48 years. The majority of women self-described their sexual orientation as heterosexual (91.2%). Most women were in a married, committed, or dating relationship, with only 21.6% describing their relationship status as single and not dating at the time of the survey.

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https://doi.org/10.1371/journal.pone.0268785.t001

Prevalence of pleasure from anal touch

Approximately four out of ten women (43.5%, n = 1,283) reported experiencing pleasure with some form of anal touch (internal or external). One quarter (25.8%) of women had never tried any form of anal touch assessed. More specifically, 27.6% of respondents reported no prior experience with external anal touch, or “not knowing” how pleasurable that type of touch might be for them; 32.5% had never tried any penetrative/internal anal touch techniques or did not know how pleasurable they might be.

Prevalence of pleasure during anal surfacing

As shown in Table 2 , 40.3% of U.S. women have used and found pleasurable Anal Surfacing, or touch on the outside of the anus. One-third (34.0%) of women have found anal surfacing using a partner’s finger pleasurable whereas about one-fifth (20.3%) have found using their own finger pleasurable. Anal surfacing using a partner’s penis was reported as pleasurable by about one-third of women (31.4%) and about one-fifth (19.4%) reported using a sex toy for this kind of touch as pleasurable. About a quarter of participants noted they did not enjoy any form of anal surfacing (22.3%) and one-third of women reported they found no pleasure in each specific anal surfacing technique (31.6%–38.5%).

https://doi.org/10.1371/journal.pone.0268785.t002

Prevalence of pleasure during anal penetration and, specifically, anal shallowing

As shown in Table 3 , 34.6% of U.S. women have used and found pleasurable touch inside of the anus. Anal penetration using a partner’s finger was the most prevalent internal touch technique, found pleasurable by about over one-quarter (28.3%) of women. Half that number of women have found using their own finger pleasurable (15.4%). Anal penetration with a partner’s penis was reported as pleasurable by nearly the same proportion (25.7%) of women who enjoyed touch by a partner’s finger. Approximately one-sixth of women (17.4%) reported using a sex toy for this kind of touch as pleasurable. About a quarter of participants noted they did not enjoy any form of touch inside the anus (23.6%) and one-third of women reported they found no pleasure in each specific touch technique (30.9%–36.0%).

https://doi.org/10.1371/journal.pone.0268785.t003

Table 4 demonstrates that 38.2% of women who had experienced pleasure from any form of anal penetration found shallower depths of Anal Shallowing pleasurable, whereas 41.6% found deeper penetration pleasurable. Out of possible penetration depths, Anal Shallowing just barely inside of the anus such as with a fingertip was reported as pleasurable by the second greatest proportion of women; one-quarter (24.7%) of those who had experienced pleasure from any form of anal penetration enjoyed this shallow depth of penetration. The slightly deeper (‘about one knuckle inside’) sub-form of Anal Shallowing we assessed was found pleasurable by 13.5% of women who had experienced pleasure from any form of anal penetration. About a quarter found a finger’s depth pleasurable and 12.9% enjoyed depths more than a finger can typically reach.

https://doi.org/10.1371/journal.pone.0268785.t004

Anal pairing and types of pleasure experienced from anal touch

Women (N = 1,283) who reported pleasure with anal surfacing and/or anal penetration (shallow or deeper) were subsequently asked about the ways in which anal touch was pleasurable for them, as summarized in Table 5 . Over one-quarter of women (27.6%) enjoy anal touch because it makes their orgasms feel more intense. The most prevalent way in which anal touch was reported as pleasurable was as a complement to other forms of touch (e.g. clitoral touch and vaginal penetration) when done simultaneously; we refer to this technique as Anal Pairing. Four out of ten (39.9%) women endorsing pleasure from any form of anal touch reported that anal touch makes other sexual touch more pleasurable for them and one-sixth (16.7%) of women specifically noted it makes reaching orgasm easier during other kinds of sexual touch.

https://doi.org/10.1371/journal.pone.0268785.t005

Prevalent ways in which women discover anal touch is pleasurable for them

For many women who currently enjoy anal touch, finding pleasure with it was a gradual process. Indeed, 56.1% of women did not find Anal Surfacing to be pleasurable the first time they tried it, but rather enjoyed it more over time. Similarly, 67.7% of women who now enjoy penetrative anal touch did not find it pleasurable at first, but rather came to enjoy it over time. As shown in Table 6 , we assessed seven patterns through which women make the initial, often gradual, discovery that anal touch is pleasurable for them. The most prevalent discovery patterns involved women’s partners. For almost half of women (44.6%), their first experience of pleasure from anal touch came as a result of a partner approaching it in a way that worked for them. Four out of ten (39.4%) women noted the importance of the emotional connection they felt with the partner with whom they were trying anal touch as a critical factor in their discovery of anal pleasure. One-quarter of women suggested that physical factors such as having enough time to become aroused beforehand (24.7%) or simply using sufficient lubricant (amount self-determined; 22.7% of women) made the biggest difference in their discovery that anal touch could feel good. To a lesser extent, women (14.0%) noted that their own self-touch during masturbation was how they discovered anal touch could be pleasurable. There was a wide range of ages (14–72 years-old) at which women discovered anal touch was pleasurable for them. The mean age of discovery, however, was the same for both internal (n = 685) and external (n = 734) anal touch: 27 years-old.

https://doi.org/10.1371/journal.pone.0268785.t006

Despite the anatomic pleasure potential [ 4 ] of the anus to promote to maximize women’s sexual enjoyment [ 2 , 3 ], the specific ways in which women might engage the anus—by themselves or with partners—as a part of their sexual repertoire has remained largely unexamined in the current sexuality literature. To address this gap, we used data from the second OMGYES Pleasure Report to providing the first detailed, population level description of the of the distinct anal stimulation and penetration techniques women engage to enhance their sexual pleasure during both solo and partnered sex. Specifically, through inductive, qualitative research followed by this nationally representative survey, we identified and named three previously undefined anal touch techniques that are found pleasurable by large proportions of U.S. women: Anal Shallowing, Anal Surfacing, and Anal Pairing.

Describing the ways in which women engage the anus in personally enjoyable ways is an important expansion of the existing anus-focused literature in several ways. One important contribution of this work is our naming of touch and stimulation techniques in lay phrasing that is usable by women and their partners. As we have suggested in our work focused on vaginal stimulation and penetration [ 24 ], for women to be comfortable communicating what new techniques they want to try and/or what existing techniques they want to try differently, the words and descriptions they use to do so must be both straightforward and accessible to them. For the vast majority of anal sexual practices, however, with some exceptions around oral-anal sexual touch (specifically referred to as analingus , rimming , a rim job or tossing the salad) and deep anal penetration with a hand or arm (specifically referred to as anal fisting , handballing or brachioproctic eroticism ) [ 31 ], there has been a lack of standalone, clear terminology. For example, the term Anal Surfacing we developed refers to touch on the outside of the anus, which 4 out of 10 women have discovered is pleasurable. The technique we named Anal Shallowing refers to touch just inside the anal opening, no deeper than a fingertip/knuckle, which was found pleasurable by 38.2% of women who enjoy any form of anal penetration. This study also identified anal touch paired with simultaneous vaginal and/or clitoral touch—referred to as Anal Pairing —can work synergistically with other forms of sexual touch as an enhancement of women’s pleasure and orgasm. Language for knowing how and for what kinds of anal touch to ask a partner may be especially important since most prevalent forms of touch reported as pleasurable for women were done by a partner’s finger or penis. The wide range of ages at which women discovered that external and/or penetrative anal touch was pleasurable to them illustrates that new pleasurable touch techniques can be discovered at any age and may encourage women to continue exploring their pleasure throughout the lifespan. Thus, there is a wider anal sexual repertoire that women enjoy in everyday life than has been named in scientific literature or that is often discussed openly in society.

In addition, we also explored the dimensionality of these techniques, identifying what is being used for touch or stimulation (e.g., own or partner’s finger, penis, or sex toy), the manner in which it is being used (e.g., on the surface vs. inside, or just barely inside vs. one knuckle in vs. deeper than a finger’s length inside), why they find it pleasurable (e.g. increased orgasm intensity vs. thrill vs. intimacy) and how they discovered it (e.g. partner introduction vs. self-exploration). This granularity supports a women-centered models of anal sexuality in which women and/or their partners are empowered to choose anal touch and stimulation technique(s) that meets their own personal pleasure needs. As suggested earlier in this paper, most of what is “known”—either through scientifically literature or through popular culture—about using the anus during sex typically focuses on the anus being penetrated by another body part [ 1 , 5 – 15 ] or emphasizes the adverse outcomes (e.g. disease, pain or coercion) associated with anal sex for women [ 16 – 18 ]. Our detailed, nationally representative data on the prevalence of women’s participation in techniques and their variants, their technique discovery, and their motivation for techniques, is an important validation for women and/or their partners that individuals like themselves can and do actively choose anal touch and stimulation to meet their own personal pleasure needs. Information such as this is vital for women’s ability to “normalize” their participation in and motivations for a behavior that is often socially stigmatized [ 19 ].

Limitations and strengths

Several limitations associated with the current data should be considered. From a measurement perspective, some survey items assessed technique participation in general, whereas others assessed technique participation in association with sexual pleasure, which could challenge disentangling a participant’s reported use of technique from their motivation for choosing that technique, as well as disentangling their actual experience of pleasure or displeasure. For example, regarding pleasure from deep penetration preference depths, we asked participants “What depth have you found anal stimulation most pleasurable?” A participant’s selection of option (e.g., “one knuckle or less”) is comparative in nature and does not necessarily mean they do not enjoy another option (e.g., “two knuckles or more”). It is possible this approach could result in an underestimate of enjoyment in these approaches. Future measurement approaches to address this challenge could include both assessing lifetime participation in specific techniques, with linking questions regarding motivation or expected pleasure, as well as actual pleasure outcomes for those who affirmed participation.

In addition, participants only included female-identified individuals; we queried men neither about their experiences with any of the anal techniques assessed here nor about how such participation may have impacted their own or their partner’s sexual pleasure. It will be important for ongoing studies to understand how selection and enjoyment of specific anal techniques are linked to partnership factors like relationship satisfaction and happiness, communication, and sexual satisfaction.

Finally, we did not assess any oral-anal behaviors as part of the larger survey informing this paper. While such behaviors are part of a larger anal sexual behavior repertoire for women [ 1 , 13 , 14 ], survey lengths precluded their inclusion. It will be important in future work for these items to be added, particularly for understanding how participation and pleasure compare to the touch and penetration behaviors we assess here. These limitations are balanced with several methodological and substantive strengths of this study. From a methodological perspective, our use of a nationally representative probability sample permits generalization of findings to the broader population of women in the United States. Other sampling approaches common in sexual and reproductive health research, including convenience, clinical or community-based recruitment, do not allow this level of comparison. In addition, our use of Ipsos’ KnowledgePanel ® affords several data collection advantages, including access to already experienced survey participants, secure survey storage and sending of participation reminders to potential respondents. Ipsos also controls the number of surveys sent to each member, minimizing the unit- and item-level missingness on any given survey. Another methodological strength is online data collection, which facilitates survey completion in a setting of the participant’s choosing, thereby increasing data confidentiality and participant comfort with answering questions about potentially sensitive topics, like sexual behavior and sexual pleasure.

Data from this U.S. nationally representative survey provide descriptions of and prevalence estimates for three techniques women have discovered to make anal stimulation and penetration more pleasurable: Anal Surfacing, Anal Shallowing, and Anal Pairing. Our findings contribute to the growth of much needed, detailed literature on the ways in which women discover, engage in, and enjoy anal stimulation and penetration. Knowledge of these techniques can enable women to better identify their own preferences, communicate about them and advocate for their sexual pleasure.

Supporting information

S1 table. definitions for and sexually explicit line drawing illustrations of anal surfacing, anal shallowing, and anal pairing techniques for experiencing sexual pleasure during anal touch..

https://doi.org/10.1371/journal.pone.0268785.s001

Acknowledgments

We would like to acknowledge Dr. Debby Herbenick for her contributions to the development of survey items for this study. We would like to thank Dr. Nicole S. Zelin for her participation in drafting and reviewing literature for the manuscript. We would also like to thank Dr. Jennifer Arter & Dr. Sybil Lockhart for their contributions to the pilot qualitative research phase, and preliminary qualitative analysis.

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